고추의 유전자적 웅성불임 유전자 msk와 연관된 분자표지 개발

Male sterility is the most important trait for F1 hybrid seed production in chili pepper (Capsicum annuum L.). Although cytoplasmic-genic male sterility (CGMS) system has advantages in aspects of cultivar development and variety protection, a genic male sterility (GMS) system has been used for producing hybrid seeds with the advantages of using diverse germplasm of different cytoplasm and restorers, stable male sterility, and short duration for cultivar development. In Korea, a GMS msk line has been used as an alternative of CGMS in some pepper breeding materials showing unstable CGMS. In this study, we aimed to develop msk-linked markers for increasing selection efficiency of maternal male sterile lines. Two F2 segregating populations (GMSK-1 and GMSK-2) were made by selfing hybrids (Mskmsk) and subjected to BSA-AFLP using 256 primer combinations (E-GNN/M-ANN). Among four reproducible polymorphic primer combinations (E-GAG/M-ACG, E-GTA/M-ACG, E-GTC/M-ACG and E-GGG/M-ATC), an AFLP marker E-GTA/M-ACG100 was converted to a codominant cleavage amplified polymorphic sequence (CAPS) marker by using PCR walking method. This marker, named GMSK-CAPS, cosegregates with msk gene with estimated linkage distance 0 cM. The marker validity has been confirmed and the marker is being used for breeding maternal lines of GMS in Korean chili pepper.