고추의 Tobamovirus 저항성 L 유전자좌와 연관된 대립유전자 특이적인 마커 세트

The resistance to Tobamovirus in Capsicum spp. has been known to be controlled by five different alleles (L0, L1, L2, L3, and L4) of L locus on the telomere of long arm of pepper chromosome 11. To develop a set of molecular markers differentiating all the alleles of L locus, we used five pepper differential hosts
including Capsicum annuum Early California Wonder (ECW, L0L0), C. annuum Tisana (L1L1), C. annuum Criollo de Morelos 334 (CM334, L2L2), Capsicum chinense PI 159236 (L3L3), and Capsicum chacoense PI 260429 (L4L4). Developing a series of CAPS or SCAR markers specifically linked to the alleles was allowed
by the sequence comparison of PCR amplicons of the L3-linked markers (189D23M, A339, and 253A1R) and BAC sequences (FJ597539 and FJ597541) in the pepper differentials. Genotypes deduced by these markers in 48 out of 53 F1 hybrids of commercial pepper varieties were consistent with their phenotypes by bioassay using Tobamovirus pathotypes (P0, P1, and P1,2). Consequently, these markers can be useful to differentiate L alleles and for breeding Tobamovirus resistance in pepper with marker-assisted selection.