고추 유전자적 웅성불임성 ms3 유전자 연관 SNP 분자표지 개발

Male sterility is used to mass-produce F1 hybrid seeds in pepper (Capsicum annuum L.). In particular, the development of genetic male sterility (GMS)-linked molecular markers may play a crucial role in hybrid breeding of pepper. To date, approximately 20 GMS genes have been identified in pepper. Among these, several molecular markers for the ms3 gene have been developed in previous studies; however, they are not completely linked and thus have limitations for use in selection. Therefore, in the present study, we aimed to develop molecular markers for ms3 selection using single-nucleotide polymorphism (SNP)-based high-resolution melting (HRM) analysis. Chi-square test was conducted using
three F2 segregating populations, and the results confirmed a 3:1 segregation ratio between male-fertile and male-sterile plants. A total of 128 primer sets were designed by selecting SNPs near the ms3 gene, and 25 HRM markers were successfully developed. Using 420 individuals from the F2 segregating population ‘GMS3,’ a high-density genetic linkage map of pepper chromosome 1 was constructed, with eight HRM markers found to be co-segregated with the ms3 gene. Subsequent experiments using various plant materials validated these eight markers, ultimately identifying two HRM markers, HRM119655681
and HRM135273656, for the final selection. These two markers showed co-segregation between the phenotype and genotype of ms3 across all plant materials used in the study. The markers developed in this study are expected to be effective for maternal line development and large-scale F1 hybrid seed production using ms3 in pepper.