쉬프 염기 가교 산화 젤란검/실크 피브로인 주사형 하이드로젤을 통한 TGF-β 3 지속 전달 및 연골 재생

As osteoarthritis rises with aging, effective strategies for non-regenerative articular cartilage are essential. We developed hydrogels crosslinked with TGF-β3 using the Schiff base reaction between oxidized gellan gum (OGG) and silk fibroin (SF). We confirmed the formation of crosslinks and the porous structure through physical and chemical property analysis. As the SF content increased, we observed a decrease in sol fraction, a decrease in swelling ratio, and an increase in compressive strength (8.2 kPa→23.5 kPa). Notably, the OGS0.5% hydrogel group effectively modulated the initial burst release of TGF-β3 compared to the free solution state and sustained release for over 28 days, promoting the proliferation of human bone marrow-derived mesenchymal stem cells and cartilage differentiation. The accumulation of GAG was 2.8 times higher, and there was increase in the expression of cartilage-specific genes like SOX9, COL2A1, and COMP. In conclusion, this hydrogel has the potential to be a promising injectable scaffold that can effectively induce cartilage regeneration.