파킨슨병 Planaria 모델에서 Gryllus bimaculatus와 Oxya chinensis sinuosa 추출물의 잠재적인 항파킨슨병 및 항산화 효과

This study evaluated the antioxidant and anti-parkinsonian effects of edible insect [Gryllus bimaculatus (Gb) and Oxya chinensis sinuosa (Ocs)] extracts, in a planarian (Dugesia japonica) model, exposed to 6-hydroxydop-amine (6-OHDA) and/or 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine hydrochloride (MPTP). The effects of Gb and Ocs, on planarian locomotor velocity (pLMV), planarian seizure-like activity (pSLA), and antioxidant activities were examined. Neither Gb nor Ocs alone had any significant effect on phenotype, pLMV, or pSLA when optimal concentrations of insect extracts were evaluated for 5 min, whereas 6-OHDA plus MPTP produced dose-dependent symptoms of parkinsonism such as phenotype alteration, decreased pLMV, and increased pSLA. In particular, planarians exposed to 6-OHDA showed only a head-bop-like pattern, not the screw-like, snake-like, or c-like patterns, whereas MPTP did not induce any seizure-like behavioral pattern. Furthermore, treatment with combined insect extracts significantly recovered the parkinsonism induced by different concentrations of 6-OHDA and MPTP by restoring, pLMV, pSLA, and phenotypic alterations. When the antioxidant effects of insect extracts were evaluated via catalase and superoxide dismutase activities, and lipid peroxidation level in planarian lysates co-treated with 6-OHDA or MPTP and Gb or Ocs, the antioxidant effects remained at the non-treated control level. Collectively, the study suggests Gb and Ocs extracts might be useful anti-parkinsonian drugs with potent antioxidant activity, and that 6-OHDA may be more useful than MPTP for anti-parkinsonian drug screening using Dugesia japonica models of parkinsonian.