파프리카 시판 품종에 대한 유전자적 웅성불임성의 대립성 및 분자표지의 유용성 검정

Paprika (Capsicum annuum L.), a colored bell-type sweet pepper, is one of the most important money making vegetable crops in Korea. The cultivation area, total production, and exports of paprika are gradually getting increased, but the paprika cultivars used in Korea are all imported. It was well-known that the genic male sterility (GMS) is the main way to produce paprika hybrid seeds. However, it is little known that how many and what kinds of ms genes are used for breeding of paprika F1 varieties. In this study, eight paprika cultivars (‘Special’, ‘Debla’, ‘Plenty’, ‘Fiero’, ‘Boogie’, ‘Fiesta’, ‘Derby’, and ‘Minibell’), popularly cultivated in Korea and three different genic male sterile lines (‘GMSP’, ‘GMS3’, and ‘GMSK’) were used. For allelism test among the F1 cultivars, half diallel crosses were performed. The result demonstrated that the most of the GMS in paprika cultivars except for ‘Minibell’ were same allele. To identify which GMS gene(s) were used for paprika F1 cultivars, top crosses between previously known GMS lines and the F1 cultivars were performed. As a result, we found that the msk and the msp genes were alleles for the GMS of ‘Minibell’ and for the other cultivars, respectively. We also confirmed that the GMS gene identification
using GMSK-CAPS marker linked to the msk gene and the PmsM1-CAPS marker linked to the msp gene in F2 progenies of ‘Minibell’ and ‘Fiesta’ and ‘Derby’ cultivars, respectively. In addition, we developed the PmsM2-CAPS marker for ‘Plenty’, ‘Fiero’, and ‘Boogie’ cultivars. We expect that these markers will be
very useful for breeding new maternal (male sterile) line of paprika.