16S rRNA molecular identification of metal tolerant bacteria isolated from electronic waste recycling facility and its heavy metal removal ability

The lead nitrate removal by isolated lead tolerant bacteria Pseudomonas sp isolated from electronic waste (e-waste) recycling facility soil was studied in a laboratory simulated environment. The bacterium was identified by biochemical and 16S rRNA molecular studies. The DNA was isolated and amplified using universal bacterial primers. The phylogeny tree suggested the isolated lead tolerant bacterium as Pseudomonas aeruginosa. The partial 16S rRNA sequences were submitted to NCBI Genbank and the eccession number JN102340 was assigned to the submitted sequences. Various parameters were optimized for lead nitrate removal such as pH, temperature, kinetics studies and metal tolerant assay. The metal removal studies suggested that the lead tolerant Pseudomonas aeruginosa JN102340 able to remove lead nitrate 92% at pH 6, 89% at 35°C, 90% after 24h incubation in the optimized pH and temperature. The bacterium Pseudomonas aeruginosa JN102340 tolerate and survive up to 600mg L^-1 lead nitrate.