3D-Printed Autologous Micronized Adipose Patch Graft for Skin Regeneration in a Dog with a Refractory Skin Defect after a Soft Tissue Sarcoma Resection

A 13-year-old neutered male Maltese dog presented with a chronic, non-healing skin defect in the right femoro-inguinal region after the wide-margin excision of a grade II soft tissue sarcoma and failed secondary intention healing. The wound remained open and inflamed for more than 60 days despite primary transposition flap closure and daily wound care with mupirocin and sugar dressings. Surgical debridement was followed by autologous fat grafting using a novel 3D-printed micronized adipose patch. The adipose tissue was harvested from the falciform ligament, mechanically processed to remove impurities, concentrated on an extracellular matrix and growth factors, and used as a bioink in a 3D bioprinter to fabricate a custom-sized patch tailored to the wound dimensions. The patch was adhered with fibrin glue and covered with a moist dressing. The graft integrated rapidly, with granulation tissue forming within three days. Complete wound closure, including hair regrowth, was achieved by day 25 without complications. This case confirmed the clinical feasibility and therapeutic potential of 3D-printed autologous micronized fat grafting for treating refractory skin wounds in dogs.