A new pancreatic lipase inhibitor isolated from the roots of Actinidia arguta

A new coumaroyl triterpene, 3-O-trans-p-coumaroyl actinidic acid (1), as well as five known triterpenes, ursolic acid (2), 23-hydroxyursolic acid (3), corosolic acid (4), asiatic acid (5) and betulinic acid (6) were isolated from an EtOAc-soluble extract of the roots of Actinidia arguta. The structure of compound 1 was elucidated from interpretation of the spectroscopic data, particularly by extensive 1D and 2D NMR studies. All the isolates (1-6) were evaluated in vitro for their inhibitory activities on pancreatic lipase (PL). Of the isolates, the new compound 1 possessed the highest inhibitory activity on PL, with an IC₅₀ of 14.95 μM, followed by ursolic acid (2, IC ₅₀ = 15.83 μM). The other four triterpenes (3-6) also showed significant PL inhibitory activity, with IC₅₀ values ranging from 20.42 to 76.45 μM.