Abstract
A colorimetric Loop-mediated isothermal Amplification (LAMP) assay with hydroxy naphthol blue was designed for the detection of Law sonia (L.) intracellularis which is an important pathogenic bacteria that causes animal diseases. A set of six primers was designed to target the aspartate ammoma-lyase (aspA) gene. Serial 10 fold dilutions of cultured L. intracellularis and spiked feces were used for LAMP optimization. The lower limit of the linear range of the assay inL. intracellularis was 101 L. intracellularis which is ten times more sensitive than conventional PCR. Based on testing in 210 porcine fecal samples using LAMP and conventional PCR, the agreement quotients between LAMP and conventional PCR was 0.92. These results suggest that colorimetric LAMP is a simple method forL. intracellularis detection in porcine fecal samples.
| Original language | English |
|---|---|
| Pages (from-to) | 4258-4262 |
| Number of pages | 5 |
| Journal | Journal of Animal and Veterinary Advances |
| Volume | 11 |
| Issue number | 22 |
| DOIs | |
| State | Published - 2012 |
Keywords
- Lamp
- Lawsonia intracellularis
- Loop-mediated isothermal amplification
- Pigs
- Porcine proliferative enteropathy
Quacquarelli Symonds(QS) Subject Topics
- Agriculture & Forestry
- Veterinary Science
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