A Signature-Tagged Mutagenesis (STM)-based murine-infectivity assay for Cryptococcus neoformans

Kwang Woo Jung; Kyung Tae Lee; Yong Sun Bahn

doi:10.1007/s12275-020-0341-8

A Signature-Tagged Mutagenesis (STM)-based murine-infectivity assay for Cryptococcus neoformans

Kwang Woo Jung
, Kyung Tae Lee
, Yong Sun Bahn^*

^*Corresponding author for this work

Korea Zoonosis Research Institute at Jeonbuk National University

Korea Atomic Energy Research Institute
Yonsei University

Research output: Contribution to journal › Journal article › peer-review

2 Scopus citations

Abstract

Signature-tagged mutagenesis (STM) is a high-throughput genetic technique that can be used to investigate the function of genes by constructing a large number of mutant strains with unique DNA identification tags, pooling them, and screening them for a particular phenotypic trait. STM was first designed for the identification of genes that contribute to the virulence or infectivity of a pathogen in its host. Recently, this method has also been applied for the identification of mutants with specific phenotypes, such as antifungal drug resistance and proliferation. In the present study, we describe an STM method for the identification of genes contributing to the infectivity of Cryptococcus neoformans using a mutant library, in which each strain was tagged with a unique DNA sequence.

Original language	English
Pages (from-to)	823-831
Number of pages	9
Journal	Journal of Microbiology
Volume	58
Issue number	10
DOIs	https://doi.org/10.1007/s12275-020-0341-8
State	Published - 2020.10.1

Keywords

fungal pathogens
mutant library
quantitative PCR
virulence

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10.1007/s12275-020-0341-8

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title = "A Signature-Tagged Mutagenesis (STM)-based murine-infectivity assay for Cryptococcus neoformans",

abstract = "Signature-tagged mutagenesis (STM) is a high-throughput genetic technique that can be used to investigate the function of genes by constructing a large number of mutant strains with unique DNA identification tags, pooling them, and screening them for a particular phenotypic trait. STM was first designed for the identification of genes that contribute to the virulence or infectivity of a pathogen in its host. Recently, this method has also been applied for the identification of mutants with specific phenotypes, such as antifungal drug resistance and proliferation. In the present study, we describe an STM method for the identification of genes contributing to the infectivity of Cryptococcus neoformans using a mutant library, in which each strain was tagged with a unique DNA sequence.",

keywords = "fungal pathogens, mutant library, quantitative PCR, virulence",

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AU - Jung, Kwang Woo

AU - Lee, Kyung Tae

AU - Bahn, Yong Sun

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Y1 - 2020/10/1

N2 - Signature-tagged mutagenesis (STM) is a high-throughput genetic technique that can be used to investigate the function of genes by constructing a large number of mutant strains with unique DNA identification tags, pooling them, and screening them for a particular phenotypic trait. STM was first designed for the identification of genes that contribute to the virulence or infectivity of a pathogen in its host. Recently, this method has also been applied for the identification of mutants with specific phenotypes, such as antifungal drug resistance and proliferation. In the present study, we describe an STM method for the identification of genes contributing to the infectivity of Cryptococcus neoformans using a mutant library, in which each strain was tagged with a unique DNA sequence.

AB - Signature-tagged mutagenesis (STM) is a high-throughput genetic technique that can be used to investigate the function of genes by constructing a large number of mutant strains with unique DNA identification tags, pooling them, and screening them for a particular phenotypic trait. STM was first designed for the identification of genes that contribute to the virulence or infectivity of a pathogen in its host. Recently, this method has also been applied for the identification of mutants with specific phenotypes, such as antifungal drug resistance and proliferation. In the present study, we describe an STM method for the identification of genes contributing to the infectivity of Cryptococcus neoformans using a mutant library, in which each strain was tagged with a unique DNA sequence.

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KW - quantitative PCR

KW - virulence

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JO - Journal of Microbiology

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ER -

A Signature-Tagged Mutagenesis (STM)-based murine-infectivity assay for Cryptococcus neoformans

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Keywords

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