Abstract
We developed a cell suspension culture system for zedoary (Curcuma zedoaria Roscoe), using 100 g fresh weight inoculum in a batch culture. The maximum cell biomass of 68.46 g/L fresh weight was obtained after 14 days of culture in a 10 L bioreactor with a pitch-blade impeller maintained at an agitation speed of 150 rpm and an aeration rate of 2.5 L/min. The accumulation of sesquiterpenes and polysaccharide in zedoary cells from 2 to 18 days was measured by HPLC and a phenol-sulfuric acid assay, respectively. The total polysaccharide concentration increased between 2 to 10 days of culture and reached a maximum value of 6.55%. HPLC revealed several eluted peaks of sesquiterpenes, which increased in amplitude from days 2 to 10. Furthermore, our results indicated that biotransformation occurred in the cell suspension, transforming certain sesquiterpenes into other types during culture.
| Original language | English |
|---|---|
| Pages (from-to) | 619-624 |
| Number of pages | 6 |
| Journal | Biotechnology and Bioprocess Engineering |
| Volume | 14 |
| Issue number | 5 |
| DOIs | |
| State | Published - 2009.10 |
Keywords
- Cell suspension
- Curcuma zedoaria
- HPLC
- Polysaccharide
- Sesquiterpenes
Quacquarelli Symonds(QS) Subject Topics
- Engineering - Chemical
- Biological Sciences
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