Acute liver toxicity by carbon tetrachloride in HSP70 knock out mice

Ji Ye Song; Lan Li; Jae Bum Ahn; Jong Gil Park; Ji Sang Jo; Dae Hun Park; Hyung Kwan Jang; Ja June Jang; Min Jae Lee

doi:10.1016/j.etp.2007.02.005

Acute liver toxicity by carbon tetrachloride in HSP70 knock out mice

Ji Ye Song
, Lan Li
, Jae Bum Ahn
, Jong Gil Park
, Ji Sang Jo
, Dae Hun Park
, Hyung Kwan Jang
, Ja June Jang
, Min Jae Lee^*

^*Corresponding author for this work

Department of Veterinary Medicine

Research output: Contribution to journal › Journal article › peer-review

33 Scopus citations

Abstract

The effects of carbon tetrachloride (CCl₄) treatment on acute liver damage in knock out (heat shock proteins-HSP70-/-) mice and wild-type (C57BL/6) mice were examined. Acute liver injury was induced by a single intraperitoneal injection of 0.3 ML/kg CCl₄ in olive oil. Mice were sacrificed at 12, 24, 48 and 72 h after treatment. To assess hepatotoxicity, alanine transaminase, neutrophil infiltration and degree of necrosis were measured. Western blot analysis was employed for heat shock proteins. The result revealed that HSP70-/- mice showed higher alanine transaminase levels and a more severe degree of neutrophilic infiltration and necrosis than those of wild-type mice. Furthermore, HSP70-/- mice recovered more slowly from CCl₄ treatment. In HSP70-/- mice, HSP47 was overexpressed. Therefore, HSP70-/- mice could be an adequate model of acute liver toxicity study.

Original language	English
Pages (from-to)	29-34
Number of pages	6
Journal	Experimental and Toxicologic Pathology
Volume	59
Issue number	1
DOIs	https://doi.org/10.1016/j.etp.2007.02.005
State	Published - 2007.09.1

Keywords

Acute liver toxicity
Carbon tetrachloride (CCl<inf>4</inf>)
HSP70
Knock out
Mice

Quacquarelli Symonds(QS) Subject Topics

Medicine
Pharmacy & Pharmacology
Biological Sciences

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10.1016/j.etp.2007.02.005

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title = "Acute liver toxicity by carbon tetrachloride in HSP70 knock out mice",

abstract = "The effects of carbon tetrachloride (CCl4) treatment on acute liver damage in knock out (heat shock proteins-HSP70-/-) mice and wild-type (C57BL/6) mice were examined. Acute liver injury was induced by a single intraperitoneal injection of 0.3 ML/kg CCl4 in olive oil. Mice were sacrificed at 12, 24, 48 and 72 h after treatment. To assess hepatotoxicity, alanine transaminase, neutrophil infiltration and degree of necrosis were measured. Western blot analysis was employed for heat shock proteins. The result revealed that HSP70-/- mice showed higher alanine transaminase levels and a more severe degree of neutrophilic infiltration and necrosis than those of wild-type mice. Furthermore, HSP70-/- mice recovered more slowly from CCl4 treatment. In HSP70-/- mice, HSP47 was overexpressed. Therefore, HSP70-/- mice could be an adequate model of acute liver toxicity study.",

keywords = "Acute liver toxicity, Carbon tetrachloride (CCl<inf>4</inf>), HSP70, Knock out, Mice",

author = "Song, \{Ji Ye\} and Lan Li and Ahn, \{Jae Bum\} and Park, \{Jong Gil\} and Jo, \{Ji Sang\} and Park, \{Dae Hun\} and Jang, \{Hyung Kwan\} and Jang, \{Ja June\} and Lee, \{Min Jae\}",

year = "2007",

month = sep,

day = "1",

doi = "10.1016/j.etp.2007.02.005",

language = "English",

volume = "59",

pages = "29--34",

journal = "Experimental and Toxicologic Pathology",

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number = "1",

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TY - JOUR

T1 - Acute liver toxicity by carbon tetrachloride in HSP70 knock out mice

AU - Song, Ji Ye

AU - Li, Lan

AU - Ahn, Jae Bum

AU - Park, Jong Gil

AU - Jo, Ji Sang

AU - Park, Dae Hun

AU - Jang, Hyung Kwan

AU - Jang, Ja June

AU - Lee, Min Jae

PY - 2007/9/1

Y1 - 2007/9/1

N2 - The effects of carbon tetrachloride (CCl4) treatment on acute liver damage in knock out (heat shock proteins-HSP70-/-) mice and wild-type (C57BL/6) mice were examined. Acute liver injury was induced by a single intraperitoneal injection of 0.3 ML/kg CCl4 in olive oil. Mice were sacrificed at 12, 24, 48 and 72 h after treatment. To assess hepatotoxicity, alanine transaminase, neutrophil infiltration and degree of necrosis were measured. Western blot analysis was employed for heat shock proteins. The result revealed that HSP70-/- mice showed higher alanine transaminase levels and a more severe degree of neutrophilic infiltration and necrosis than those of wild-type mice. Furthermore, HSP70-/- mice recovered more slowly from CCl4 treatment. In HSP70-/- mice, HSP47 was overexpressed. Therefore, HSP70-/- mice could be an adequate model of acute liver toxicity study.

AB - The effects of carbon tetrachloride (CCl4) treatment on acute liver damage in knock out (heat shock proteins-HSP70-/-) mice and wild-type (C57BL/6) mice were examined. Acute liver injury was induced by a single intraperitoneal injection of 0.3 ML/kg CCl4 in olive oil. Mice were sacrificed at 12, 24, 48 and 72 h after treatment. To assess hepatotoxicity, alanine transaminase, neutrophil infiltration and degree of necrosis were measured. Western blot analysis was employed for heat shock proteins. The result revealed that HSP70-/- mice showed higher alanine transaminase levels and a more severe degree of neutrophilic infiltration and necrosis than those of wild-type mice. Furthermore, HSP70-/- mice recovered more slowly from CCl4 treatment. In HSP70-/- mice, HSP47 was overexpressed. Therefore, HSP70-/- mice could be an adequate model of acute liver toxicity study.

KW - Acute liver toxicity

KW - Carbon tetrachloride (CCl<inf>4</inf>)

KW - HSP70

KW - Knock out

KW - Mice

UR - https://www.scopus.com/pages/publications/34547732373

U2 - 10.1016/j.etp.2007.02.005

DO - 10.1016/j.etp.2007.02.005

M3 - Journal article

C2 - 17582750

AN - SCOPUS:34547732373

SN - 0940-2993

VL - 59

SP - 29

EP - 34

JO - Experimental and Toxicologic Pathology

JF - Experimental and Toxicologic Pathology

IS - 1

ER -

Acute liver toxicity by carbon tetrachloride in HSP70 knock out mice

Abstract

Keywords

Quacquarelli Symonds(QS) Subject Topics

Access to Document

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