Aminipila terrae sp. nov., a strictly anaerobic bacterium isolated from river sediment

In this study, aimed at investigating and characterizing river sediment bacteria, we isolated a Gram-stain-positive, rod-shaped, obligate anaerobic bacterium, strain CBA3637^T, from the sediment of the Geum River. This strain grew at 10–40 °C (optimum, 30 °C), 0–1% NaCl (optimum, 0%), and pH 7–8 (optimum, pH 7). The 16S rRNA gene sequence comparison revealed Aminipila butyrica DSM 103574^T to be the closest relative of strain CBA3637^T (96.6–96.7% similarity); and both strains clustered together in phylogenetic analysis. The genome of strain CBA3637^T was found to consist of a single chromosome (3.51 Mbp; 36.98% G + C content). Comparative genomic analysis of the strain CBA3637^T with A. butyrica DSM 103574^T revealed that strain CBA3637^T possessed five unique pathways related to polyamine biosynthesis, lipopolysaccharide metabolism, pyrimidine metabolism, and cofactor and vitamin metabolism. Strain CBA3637^T contained C_14:0, C_16:0, and C_18:1ω9c as the major fatty acids, and diphosphatidylglycerol as the major polar lipid. No respiratory quinone was observed. Biochemical, chemotaxonomic, and genotypic data revealed that the strain CBA3637^T is a representative of a novel species within the genus Aminipila, for which the name Aminipila terrae is proposed. The type strain is CBA3637^T (= KACC 21651^T = DSM 110662^T).