An ellipsometric surface plasmon resonance sensor for the direct monitoring of small-molecular-weight analytes

  • Hyun Mo Cho*
  • , Won Chegal
  • , Yong Jai Cho
  • , Jong Myoung Won
  • , Moonseob Jin
  • , Daesuk Kim
  • *Corresponding author for this work

Research output: Contribution to conferenceConference paperpeer-review

Abstract

We report on a high-sensitivity surface plasmon resonance (SPR) sensor based on phase measurements using rotating analyzer ellipsometry. In this work, the experimental setup for the SPR sensor was based on a custom-built rotating analyzer ellipsometer, which was equipped with a SPR cell and a microfluidic system. The most important feature of our system is that biomolecular detection sensitivity can be significantly enhanced by measuring the phase difference between the s- and ppolarizations. In this paper, we demonstrate how a custombuilt rotating analyzer ellipsometer in the SPR condition can be used to directly detect the interaction of D-biotin and protein tyrosine phosphatase (PTP) inhibitor with immobilized streptavidin and PTP maltose binding protein (MBP)-SHP2.

Original languageEnglish
Title of host publication20th IMEKO World Congress 2012
Pages1614-1619
Number of pages6
StatePublished - 2012
Event20th IMEKO World Congress 2012 - Busan, Korea, Republic of
Duration: 2012.09.92012.09.14

Publication series

Name20th IMEKO World Congress 2012
Volume3

Conference

Conference20th IMEKO World Congress 2012
Country/TerritoryKorea, Republic of
CityBusan
Period12.09.912.09.14

Keywords

  • Biomolecular interactions
  • Ellipsomtry
  • Low-molecular-weight analytes
  • Surface plasmon resonance

Quacquarelli Symonds(QS) Subject Topics

  • Environmental Sciences
  • Computer Science & Information Systems
  • Data Science
  • Biological Sciences

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