@inbook{f541ac12a81e4e988ab758eae105031b,
title = "Analysis of regulatory B cells in experimental autoimmune uveitis",
abstract = "Regulatory B cells (Bregs) that produce IL-35 and IL-10 (i35-Bregs) regulate central nervous system (CNS) autoimmune diseases including uveitis. In the mouse model of uveitis, i35-Breg cells suppress intraocular inflammation by inducing expansion of IL-10-producing B cells (B10), IL-10-producing T cells (Tregs), and IL-35-producing T cells (iTR35), suggesting that i35-Bregs orchestrate an immune-suppressive milieu that regulates immunity during autoimmune diseases. In this chapter, we discuss uveitis and therapeutic challenges that necessitate the development of cell-based therapies for the treatment of these potentially blinding diseases that cause 10\% visual handicap. We then describe the methods we set up for ex vivo generation of i35-Breg cells employed in i35-Breg immunotherapy in uveitis and in other CNS autoimmune diseases.",
keywords = "CNS autoimmune disease, EAU, i35-Breg, IL-10, IL-35, Regulatory B cells, Uveitis",
author = "Choi, \{Jin Kyeong\} and Egwuagu, \{Charles E.\}",
note = "Publisher Copyright: {\textcopyright} Springer Science+Business Media, LLC, part of Springer Nature 2021.",
year = "2021",
doi = "10.1007/978-1-0716-1237-8\_23",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "437--450",
booktitle = "Methods in Molecular Biology",
}