Abstract
Human antimicrobial peptide CAP18/LL37 (hCAP18/LL37) was expressed in Pichia pastoris and its antibacterial activity was tested against pathogenic bacteria. The full length ORF of hCAP18/LL37 was cloned into the pPICZaA vector followed by integration into the genomic AOX1 gene of P. pastoris. Agar diffusion assay demonstrated that the different hCAP18/LL37 transformants showed various antibacterial activities against Staphylococcus aureus, Micrococcus luteus, and Salmonella gastroenteritis. The secreted form of hCAP18/LL37 exhibited its maximum activity after 72 h incubation with 2% methanol in MM media, not in BMM. This result suggests that the yeast secreted expression system can be used as a production tool of antimicrobial peptides for industrial or pharmaceutical application.
| Original language | English |
|---|---|
| Pages (from-to) | 358-362 |
| Number of pages | 5 |
| Journal | Journal of Microbiology |
| Volume | 47 |
| Issue number | 3 |
| DOIs | |
| State | Published - 2009 |
Keywords
- Antibacterial activity
- Antimicrobial peptide (AMP)
- hCAP18/ LL37
- Pichia pastoris
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