Anticancer activity of biologically synthesized silver and gold nanoparticles on mouse myoblast cancer cells and their toxicity against embryonic zebrafish

Rajan Ramachandran; Chandran Krishnaraj; Allur Subramaniyan Sivakumar; Palaniappan Prasannakumar; V. K. Abhay Kumar; Kwan Seob Shim; Chul Gyu Song; Soon Il Yun

doi:10.1016/j.msec.2016.12.110

Anticancer activity of biologically synthesized silver and gold nanoparticles on mouse myoblast cancer cells and their toxicity against embryonic zebrafish

Rajan Ramachandran
, Chandran Krishnaraj
, Allur Subramaniyan Sivakumar
, Palaniappan Prasannakumar
, V. K. Abhay Kumar
, Kwan Seob Shim
, Chul Gyu Song
, Soon Il Yun^*

^*Corresponding author for this work

Research output: Contribution to journal › Journal article › peer-review

75 Scopus citations

Abstract

The aim of this study was to evaluate the anticancer activity of bioinspired silver nanoparticles (AgNPs) and gold nanoparticles (AuNPs) against mouse myoblast cancer cells (C₂C₁₂). Both AgNPs and AuNPs were biologically synthesized using Spinacia oleracea Linn., aqueous leaves extract. UV–Vis. spectrophotometer, high resolution-transmission electron microscopy (HR-TEM), field emission-scanning electron microscopy (FE-SEM) and X-ray diffraction (XRD) studies supported the successful synthesis of AgNPs and AuNPs. Both these NPs have shown cytotoxicity against C₂C₁₂ cells even at very low concentration (5 μg/mL). Acridine orange/Ethidium bromide (AO/EB) dual staining confirmed the apoptotic morphological features. The levels of caspase enzymes (caspase-3 and caspase-7) were significantly up-regulated in NPs treated myoblast cells than the plant extract. Furthermore, in zebrafish embryo toxicity study, AgNPs showed 100% mortality at 3 μg/mL concentration while AuNPs exhibited the same at much higher concentration (300 mg/mL). Taken together, these results provide a preliminary guidance for the development of biomaterials based drugs to fight against the fatal diseases for example cancer.

Original language	English
Pages (from-to)	674-683
Number of pages	10
Journal	Materials Science and Engineering C
Volume	73
DOIs	https://doi.org/10.1016/j.msec.2016.12.110
State	Published - 2017.04.1

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Cytotoxicity
Gold nanoparticles
Mouse myoblast cells
Silver nanoparticles
Spinacia oleracea
Zebrafish embryo

Quacquarelli Symonds(QS) Subject Topics

Materials Science
Medicine
Engineering - Chemical

Access to Document

10.1016/j.msec.2016.12.110

Cite this

Ramachandran, R., Krishnaraj, C., Sivakumar, A. S., Prasannakumar, P., Abhay Kumar, V. K., Shim, K. S., Song, C. G., & Yun, S. I. (2017). Anticancer activity of biologically synthesized silver and gold nanoparticles on mouse myoblast cancer cells and their toxicity against embryonic zebrafish. Materials Science and Engineering C, 73, 674-683. https://doi.org/10.1016/j.msec.2016.12.110

@article{e6f6812987eb4073a0ab82fa5793aff9,

title = "Anticancer activity of biologically synthesized silver and gold nanoparticles on mouse myoblast cancer cells and their toxicity against embryonic zebrafish",

abstract = "The aim of this study was to evaluate the anticancer activity of bioinspired silver nanoparticles (AgNPs) and gold nanoparticles (AuNPs) against mouse myoblast cancer cells (C2C12). Both AgNPs and AuNPs were biologically synthesized using Spinacia oleracea Linn., aqueous leaves extract. UV–Vis. spectrophotometer, high resolution-transmission electron microscopy (HR-TEM), field emission-scanning electron microscopy (FE-SEM) and X-ray diffraction (XRD) studies supported the successful synthesis of AgNPs and AuNPs. Both these NPs have shown cytotoxicity against C2C12 cells even at very low concentration (5 μg/mL). Acridine orange/Ethidium bromide (AO/EB) dual staining confirmed the apoptotic morphological features. The levels of caspase enzymes (caspase-3 and caspase-7) were significantly up-regulated in NPs treated myoblast cells than the plant extract. Furthermore, in zebrafish embryo toxicity study, AgNPs showed 100\% mortality at 3 μg/mL concentration while AuNPs exhibited the same at much higher concentration (300 mg/mL). Taken together, these results provide a preliminary guidance for the development of biomaterials based drugs to fight against the fatal diseases for example cancer.",

keywords = "Cytotoxicity, Gold nanoparticles, Mouse myoblast cells, Silver nanoparticles, Spinacia oleracea, Zebrafish embryo",

author = "Rajan Ramachandran and Chandran Krishnaraj and Sivakumar, \{Allur Subramaniyan\} and Palaniappan Prasannakumar and \{Abhay Kumar\}, \{V. K.\} and Shim, \{Kwan Seob\} and Song, \{Chul Gyu\} and Yun, \{Soon Il\}",

note = "Publisher Copyright: {\textcopyright} 2016 Elsevier B.V.",

year = "2017",

month = apr,

day = "1",

doi = "10.1016/j.msec.2016.12.110",

language = "English",

volume = "73",

pages = "674--683",

journal = "Materials Science and Engineering C",

issn = "0928-4931",

}

Ramachandran, R, Krishnaraj, C, Sivakumar, AS, Prasannakumar, P, Abhay Kumar, VK, Shim, KS , Song, CG & Yun, SI 2017, 'Anticancer activity of biologically synthesized silver and gold nanoparticles on mouse myoblast cancer cells and their toxicity against embryonic zebrafish', Materials Science and Engineering C, vol. 73, pp. 674-683. https://doi.org/10.1016/j.msec.2016.12.110

Anticancer activity of biologically synthesized silver and gold nanoparticles on mouse myoblast cancer cells and their toxicity against embryonic zebrafish. / Ramachandran, Rajan; Krishnaraj, Chandran; Sivakumar, Allur Subramaniyan et al.
In: Materials Science and Engineering C, Vol. 73, 01.04.2017, p. 674-683.

Research output: Contribution to journal › Journal article › peer-review

TY - JOUR

T1 - Anticancer activity of biologically synthesized silver and gold nanoparticles on mouse myoblast cancer cells and their toxicity against embryonic zebrafish

AU - Ramachandran, Rajan

AU - Krishnaraj, Chandran

AU - Sivakumar, Allur Subramaniyan

AU - Prasannakumar, Palaniappan

AU - Abhay Kumar, V. K.

AU - Shim, Kwan Seob

AU - Song, Chul Gyu

AU - Yun, Soon Il

PY - 2017/4/1

Y1 - 2017/4/1

N2 - The aim of this study was to evaluate the anticancer activity of bioinspired silver nanoparticles (AgNPs) and gold nanoparticles (AuNPs) against mouse myoblast cancer cells (C2C12). Both AgNPs and AuNPs were biologically synthesized using Spinacia oleracea Linn., aqueous leaves extract. UV–Vis. spectrophotometer, high resolution-transmission electron microscopy (HR-TEM), field emission-scanning electron microscopy (FE-SEM) and X-ray diffraction (XRD) studies supported the successful synthesis of AgNPs and AuNPs. Both these NPs have shown cytotoxicity against C2C12 cells even at very low concentration (5 μg/mL). Acridine orange/Ethidium bromide (AO/EB) dual staining confirmed the apoptotic morphological features. The levels of caspase enzymes (caspase-3 and caspase-7) were significantly up-regulated in NPs treated myoblast cells than the plant extract. Furthermore, in zebrafish embryo toxicity study, AgNPs showed 100% mortality at 3 μg/mL concentration while AuNPs exhibited the same at much higher concentration (300 mg/mL). Taken together, these results provide a preliminary guidance for the development of biomaterials based drugs to fight against the fatal diseases for example cancer.

AB - The aim of this study was to evaluate the anticancer activity of bioinspired silver nanoparticles (AgNPs) and gold nanoparticles (AuNPs) against mouse myoblast cancer cells (C2C12). Both AgNPs and AuNPs were biologically synthesized using Spinacia oleracea Linn., aqueous leaves extract. UV–Vis. spectrophotometer, high resolution-transmission electron microscopy (HR-TEM), field emission-scanning electron microscopy (FE-SEM) and X-ray diffraction (XRD) studies supported the successful synthesis of AgNPs and AuNPs. Both these NPs have shown cytotoxicity against C2C12 cells even at very low concentration (5 μg/mL). Acridine orange/Ethidium bromide (AO/EB) dual staining confirmed the apoptotic morphological features. The levels of caspase enzymes (caspase-3 and caspase-7) were significantly up-regulated in NPs treated myoblast cells than the plant extract. Furthermore, in zebrafish embryo toxicity study, AgNPs showed 100% mortality at 3 μg/mL concentration while AuNPs exhibited the same at much higher concentration (300 mg/mL). Taken together, these results provide a preliminary guidance for the development of biomaterials based drugs to fight against the fatal diseases for example cancer.

KW - Cytotoxicity

KW - Gold nanoparticles

KW - Mouse myoblast cells

KW - Silver nanoparticles

KW - Spinacia oleracea

KW - Zebrafish embryo

UR - https://www.scopus.com/pages/publications/85008354400

U2 - 10.1016/j.msec.2016.12.110

DO - 10.1016/j.msec.2016.12.110

M3 - Journal article

C2 - 28183660

AN - SCOPUS:85008354400

SN - 0928-4931

VL - 73

SP - 674

EP - 683

JO - Materials Science and Engineering C

JF - Materials Science and Engineering C

ER -

Anticancer activity of biologically synthesized silver and gold nanoparticles on mouse myoblast cancer cells and their toxicity against embryonic zebrafish

Abstract

UN SDGs

Keywords

Quacquarelli Symonds(QS) Subject Topics

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