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Antioxidant and Antihyperlipidemic Effects of Isolated Soybean Protein Ferments Using Bacillus spp.

  • Hyun Wang Kim
  • , Hyeon Hwa Oh
  • , Do Youn Jeong
  • , Geun Seoup Song*
  • *Corresponding author for this work
  • Jeonbuk National University
  • Microbial Institute for Fermentation Industry (MIFI)

Research output: Contribution to journalJournal articlepeer-review

Abstract

This study investigated the functional properties, antioxidant, antihypertensive, hypolipidemic, and hypocholesterolemic effects of isolated soybean protein (ISP) fermented products using Bacillus amyloliquefaciens SRCM118817 and B. velezensis SRCM117829 in a minimal medium supplemented with 2% (w/v) ISP. The antioxidant activity of ISP ferments (AISP24h, AISP48h, VISP24h, and VISP48h) tends to increase as the fermentation period increases. The angiotensin conversion enzyme 1 (ACE1) inhibitory activity was the highest in 1/5 dilution factor (DF) of VISP24h (87.93%) among the ISP ferments. The hypolipidemic and hypocholesterolemic effects of ISP ferments were evaluated on lipid accumulation and total cholesterol contents using oleic acid (400 μM) stimulated HepG2 cells. AISP24h (56.00%) and AISP48h (55.19%) showed significantly higher lipid accumulation inhibition rates in HepG2 cells compared to VISP24h (53.10%) and VISP48h (53.78%) at 1/50 DF. Results of the analysis, indicate that at 1/100 DF and 1/50 DF, AISP48h demonstrates the most effective total cholesterol production inhibition rates in HepG2 cells at 26.86 μg/mL and 21.40 μg/mL, respectively.

Original languageEnglish
Pages (from-to)886-896
Number of pages11
JournalJournal of the Korean Society of Food Science and Nutrition
Volume52
Issue number9
DOIs
StatePublished - 2023.09

Keywords

  • antihyperlipidemic effect
  • antioxidant activity
  • isolated soybean peptide
  • lipid accumulation

Quacquarelli Symonds(QS) Subject Topics

  • Nursing
  • Agriculture & Forestry

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