Application of DNA microarray for screening metagenome library clones.

Soo Je Park; Jong Chan Chae; Sung Keun Rhee

doi:10.1007/978-1-60761-823-2_22

Application of DNA microarray for screening metagenome library clones.

Soo Je Park^*
, Jong Chan Chae
, Sung Keun Rhee

^*Corresponding author for this work

Bioresource and Biomolecule Engineering

Chungbuk National University

Research output: Contribution to journal › Journal article › peer-review

7 Scopus citations

Abstract

Sequence-based screening tools of a metagenome library can expedite metagenome researches considering tremendous metagenome diversities. Several critical disadvantages of activity-based screening of metagenome libraries could be overcome by sequence-based screening approaches. DNA microarray technology widely used for monitoring environmental genes can be employed for screening environmental fosmid and BAC clones harboring target genes due to its high throughput nature. DNAs of fosmid clones are extracted and spotted on a glass slide and fluorescence-labeled probes are hybridized to the microarray. Specific hybridization signals can be obtained only for the fosmid clones that contain the target gene with high sensitivity (10 ng/μL of fosmid clone DNA) and quantitativeness.

Original language	English
Pages (from-to)	313-324
Number of pages	12
Journal	Methods in molecular biology (Clifton, N.J.)
Volume	668
DOIs	https://doi.org/10.1007/978-1-60761-823-2_22
State	Published - 2010

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10.1007/978-1-60761-823-2_22

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title = "Application of DNA microarray for screening metagenome library clones.",

abstract = "Sequence-based screening tools of a metagenome library can expedite metagenome researches considering tremendous metagenome diversities. Several critical disadvantages of activity-based screening of metagenome libraries could be overcome by sequence-based screening approaches. DNA microarray technology widely used for monitoring environmental genes can be employed for screening environmental fosmid and BAC clones harboring target genes due to its high throughput nature. DNAs of fosmid clones are extracted and spotted on a glass slide and fluorescence-labeled probes are hybridized to the microarray. Specific hybridization signals can be obtained only for the fosmid clones that contain the target gene with high sensitivity (10 ng/μL of fosmid clone DNA) and quantitativeness.",

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AU - Park, Soo Je

AU - Chae, Jong Chan

AU - Rhee, Sung Keun

PY - 2010

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N2 - Sequence-based screening tools of a metagenome library can expedite metagenome researches considering tremendous metagenome diversities. Several critical disadvantages of activity-based screening of metagenome libraries could be overcome by sequence-based screening approaches. DNA microarray technology widely used for monitoring environmental genes can be employed for screening environmental fosmid and BAC clones harboring target genes due to its high throughput nature. DNAs of fosmid clones are extracted and spotted on a glass slide and fluorescence-labeled probes are hybridized to the microarray. Specific hybridization signals can be obtained only for the fosmid clones that contain the target gene with high sensitivity (10 ng/μL of fosmid clone DNA) and quantitativeness.

AB - Sequence-based screening tools of a metagenome library can expedite metagenome researches considering tremendous metagenome diversities. Several critical disadvantages of activity-based screening of metagenome libraries could be overcome by sequence-based screening approaches. DNA microarray technology widely used for monitoring environmental genes can be employed for screening environmental fosmid and BAC clones harboring target genes due to its high throughput nature. DNAs of fosmid clones are extracted and spotted on a glass slide and fluorescence-labeled probes are hybridized to the microarray. Specific hybridization signals can be obtained only for the fosmid clones that contain the target gene with high sensitivity (10 ng/μL of fosmid clone DNA) and quantitativeness.

UR - https://www.scopus.com/pages/publications/79952118595

U2 - 10.1007/978-1-60761-823-2_22

DO - 10.1007/978-1-60761-823-2_22

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AN - SCOPUS:79952118595

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SP - 313

EP - 324

JO - Methods in molecular biology (Clifton, N.J.)

JF - Methods in molecular biology (Clifton, N.J.)

ER -

Application of DNA microarray for screening metagenome library clones.

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