Application of proteomics for comparison of proteome of Neospora caninum and Toxoplasma gondii tachyzoites

Eung Goo Lee; Jae Hoon Kim; Yong Seung Shin; Gee Wook Shin; Young Rim Kim; K. J. Palaksha; Dae Yong Kim; Itsuro Yamane; Yong Hwan Kim; Gon Sup Kim; Myung Deuk Suh; Tae Sung Jung

doi:10.1016/j.jchromb.2004.08.048

Application of proteomics for comparison of proteome of Neospora caninum and Toxoplasma gondii tachyzoites

Eung Goo Lee
, Jae Hoon Kim
, Yong Seung Shin
, Gee Wook Shin
, Young Rim Kim
, K. J. Palaksha
, Dae Yong Kim
, Itsuro Yamane
, Yong Hwan Kim
, Gon Sup Kim
, Myung Deuk Suh
, Tae Sung Jung^*

^*Corresponding author for this work

Department of Veterinary Medicine

Sungkyunkwan University
Jeju National University
Gyeongsang National University
Seoul National University
National Agriculture and Food Research Organization

Research output: Contribution to journal › Journal article › peer-review

30 Scopus citations

Abstract

Protein profiles of two isolates of Neospora caninum (KBA-2 and JPA1) and Toxoplasma gondii RH strain were investigated by proteomic approach. Approximately, 78% of protein spots on two-dimensional gel electrophoresis (2-DE) profiles and 80% of antigen spots on 2-DE immunoblotting profiles were exhibited to share the same pI and M_r between KBA-2 and JPA1 of N. caninum. On the other hand, a total of 30 antigen spots of T. gondii were recognized on 2-DE immunoblotting profile using rabbit antiserum against N. caninum KBA-2. A number of homologue proteins, such as heat shock protein 70, tubulin α- and β-chain, putative protein disulfide isomerase, actin, enolase and 14-3-3 protein homologue are believed as the conserved proteins in both N. caninum and T. gondii. On the contrary, NcSUB1, NcGRA2 and NCDG1 (NcGRA7) might be the species-specific proteins for N. caninum tachyzoites. The present study showed that the high degree of similarity between N. caninum isolates (KBA-2 and JPA1), whereas large differences between N. caninum and T. gondii were noticed by proteome comparisons.

Original language	English
Pages (from-to)	305-314
Number of pages	10
Journal	Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume	815
Issue number	1-2
DOIs	https://doi.org/10.1016/j.jchromb.2004.08.048
State	Published - 2005.02.5

Keywords

Antigen spots
Mass spectrometry
Neospora caninum
Proteome analysis
Toxoplasma gondii
Two-dimensional gel electrophoresis

Access to Document

10.1016/j.jchromb.2004.08.048

Cite this

Lee, E. G., Kim, J. H., Shin, Y. S., Shin, G. W., Kim, Y. R., Palaksha, K. J., Kim, D. Y., Yamane, I., Kim, Y. H., Kim, G. S., Suh, M. D., & Jung, T. S. (2005). Application of proteomics for comparison of proteome of Neospora caninum and Toxoplasma gondii tachyzoites. Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, 815(1-2), 305-314. https://doi.org/10.1016/j.jchromb.2004.08.048

@article{0efa9f8ef5e04883871186301bd515a8,

title = "Application of proteomics for comparison of proteome of Neospora caninum and Toxoplasma gondii tachyzoites",

abstract = "Protein profiles of two isolates of Neospora caninum (KBA-2 and JPA1) and Toxoplasma gondii RH strain were investigated by proteomic approach. Approximately, 78\% of protein spots on two-dimensional gel electrophoresis (2-DE) profiles and 80\% of antigen spots on 2-DE immunoblotting profiles were exhibited to share the same pI and Mr between KBA-2 and JPA1 of N. caninum. On the other hand, a total of 30 antigen spots of T. gondii were recognized on 2-DE immunoblotting profile using rabbit antiserum against N. caninum KBA-2. A number of homologue proteins, such as heat shock protein 70, tubulin α- and β-chain, putative protein disulfide isomerase, actin, enolase and 14-3-3 protein homologue are believed as the conserved proteins in both N. caninum and T. gondii. On the contrary, NcSUB1, NcGRA2 and NCDG1 (NcGRA7) might be the species-specific proteins for N. caninum tachyzoites. The present study showed that the high degree of similarity between N. caninum isolates (KBA-2 and JPA1), whereas large differences between N. caninum and T. gondii were noticed by proteome comparisons.",

keywords = "Antigen spots, Mass spectrometry, Neospora caninum, Proteome analysis, Toxoplasma gondii, Two-dimensional gel electrophoresis",

author = "Lee, \{Eung Goo\} and Kim, \{Jae Hoon\} and Shin, \{Yong Seung\} and Shin, \{Gee Wook\} and Kim, \{Young Rim\} and Palaksha, \{K. J.\} and Kim, \{Dae Yong\} and Itsuro Yamane and Kim, \{Yong Hwan\} and Kim, \{Gon Sup\} and Suh, \{Myung Deuk\} and Jung, \{Tae Sung\}",

year = "2005",

month = feb,

day = "5",

doi = "10.1016/j.jchromb.2004.08.048",

language = "English",

volume = "815",

pages = "305--314",

journal = "Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences",

issn = "1570-0232",

number = "1-2",

}

Lee, EG, Kim, JH, Shin, YS, Shin, GW, Kim, YR, Palaksha, KJ, Kim, DY, Yamane, I, Kim, YH, Kim, GS, Suh, MD & Jung, TS 2005, 'Application of proteomics for comparison of proteome of Neospora caninum and Toxoplasma gondii tachyzoites', Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, vol. 815, no. 1-2, pp. 305-314. https://doi.org/10.1016/j.jchromb.2004.08.048

Application of proteomics for comparison of proteome of Neospora caninum and Toxoplasma gondii tachyzoites. / Lee, Eung Goo; Kim, Jae Hoon; Shin, Yong Seung et al.
In: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, Vol. 815, No. 1-2, 05.02.2005, p. 305-314.

Research output: Contribution to journal › Journal article › peer-review

TY - JOUR

T1 - Application of proteomics for comparison of proteome of Neospora caninum and Toxoplasma gondii tachyzoites

AU - Lee, Eung Goo

AU - Kim, Jae Hoon

AU - Shin, Yong Seung

AU - Shin, Gee Wook

AU - Kim, Young Rim

AU - Palaksha, K. J.

AU - Kim, Dae Yong

AU - Yamane, Itsuro

AU - Kim, Yong Hwan

AU - Kim, Gon Sup

AU - Suh, Myung Deuk

AU - Jung, Tae Sung

PY - 2005/2/5

Y1 - 2005/2/5

N2 - Protein profiles of two isolates of Neospora caninum (KBA-2 and JPA1) and Toxoplasma gondii RH strain were investigated by proteomic approach. Approximately, 78% of protein spots on two-dimensional gel electrophoresis (2-DE) profiles and 80% of antigen spots on 2-DE immunoblotting profiles were exhibited to share the same pI and Mr between KBA-2 and JPA1 of N. caninum. On the other hand, a total of 30 antigen spots of T. gondii were recognized on 2-DE immunoblotting profile using rabbit antiserum against N. caninum KBA-2. A number of homologue proteins, such as heat shock protein 70, tubulin α- and β-chain, putative protein disulfide isomerase, actin, enolase and 14-3-3 protein homologue are believed as the conserved proteins in both N. caninum and T. gondii. On the contrary, NcSUB1, NcGRA2 and NCDG1 (NcGRA7) might be the species-specific proteins for N. caninum tachyzoites. The present study showed that the high degree of similarity between N. caninum isolates (KBA-2 and JPA1), whereas large differences between N. caninum and T. gondii were noticed by proteome comparisons.

AB - Protein profiles of two isolates of Neospora caninum (KBA-2 and JPA1) and Toxoplasma gondii RH strain were investigated by proteomic approach. Approximately, 78% of protein spots on two-dimensional gel electrophoresis (2-DE) profiles and 80% of antigen spots on 2-DE immunoblotting profiles were exhibited to share the same pI and Mr between KBA-2 and JPA1 of N. caninum. On the other hand, a total of 30 antigen spots of T. gondii were recognized on 2-DE immunoblotting profile using rabbit antiserum against N. caninum KBA-2. A number of homologue proteins, such as heat shock protein 70, tubulin α- and β-chain, putative protein disulfide isomerase, actin, enolase and 14-3-3 protein homologue are believed as the conserved proteins in both N. caninum and T. gondii. On the contrary, NcSUB1, NcGRA2 and NCDG1 (NcGRA7) might be the species-specific proteins for N. caninum tachyzoites. The present study showed that the high degree of similarity between N. caninum isolates (KBA-2 and JPA1), whereas large differences between N. caninum and T. gondii were noticed by proteome comparisons.

KW - Antigen spots

KW - Mass spectrometry

KW - Neospora caninum

KW - Proteome analysis

KW - Toxoplasma gondii

KW - Two-dimensional gel electrophoresis

UR - https://www.scopus.com/pages/publications/12344314262

U2 - 10.1016/j.jchromb.2004.08.048

DO - 10.1016/j.jchromb.2004.08.048

M3 - Journal article

C2 - 15652819

AN - SCOPUS:12344314262

SN - 1570-0232

VL - 815

SP - 305

EP - 314

JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

IS - 1-2

ER -

Application of proteomics for comparison of proteome of Neospora caninum and Toxoplasma gondii tachyzoites

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this