Aptamer-Based Paper Strip Sensor for Detecting Vibrio fischeri

Woo Ri Shin; Simranjeet Singh Sekhon; Sung Keun Rhee; Jung Ho Ko; Ji Young Ahn; Jiho Min; Yang Hoon Kim

doi:10.1021/acscombsci.7b00190

Aptamer-Based Paper Strip Sensor for Detecting Vibrio fischeri

Woo Ri Shin
, Simranjeet Singh Sekhon
, Sung Keun Rhee
, Jung Ho Ko
, Ji Young Ahn^*
, Jiho Min
, Yang Hoon Kim

^*Corresponding author for this work

Division of Chemical Engineering

Chungbuk National University
Western University of Health Sciences

Research output: Contribution to journal › Journal article › peer-review

54 Scopus citations

Abstract

Aptamer-based paper strip sensor for detecting Vibrio fischeri was developed. Our method was based on the aptamer sandwich assay between whole live cells, V. fischeri and DNA aptamer probes. Following 9 rounds of Cell-SELEX and one of the negative-SELEX, V. fischeri Cell Aptamer (VFCA)-02 and -03 were isolated, with the former showing approximately 10-fold greater avidity (in the subnanomolar range) for the target cells when arrayed on a surface. The colorimetric response of a paper sensor based on VFCA-02 was linear in the range of 4 × 10¹ to 4 × 10⁵ CFU/mL of target cell by using scanning reader. The linear regression correlation coefficient (R²) was 0.9809. This system shows promise for use in aptamer-conjugated gold nanoparticle probes in paper strip format for in-field detection of marine bioindicating bacteria.

Original language	English
Pages (from-to)	261-268
Number of pages	8
Journal	ACS Combinatorial Science
Volume	20
Issue number	5
DOIs	https://doi.org/10.1021/acscombsci.7b00190
State	Published - 2018.05.14

Keywords

aptamer-based paper strip sensor
ssDNA aptamer
Vibrio fischeri

Quacquarelli Symonds(QS) Subject Topics

Chemistry

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10.1021/acscombsci.7b00190

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@article{6c46e4e866dd4b55ad3d45d187df5fbf,

title = "Aptamer-Based Paper Strip Sensor for Detecting Vibrio fischeri",

abstract = "Aptamer-based paper strip sensor for detecting Vibrio fischeri was developed. Our method was based on the aptamer sandwich assay between whole live cells, V. fischeri and DNA aptamer probes. Following 9 rounds of Cell-SELEX and one of the negative-SELEX, V. fischeri Cell Aptamer (VFCA)-02 and -03 were isolated, with the former showing approximately 10-fold greater avidity (in the subnanomolar range) for the target cells when arrayed on a surface. The colorimetric response of a paper sensor based on VFCA-02 was linear in the range of 4 × 101 to 4 × 105 CFU/mL of target cell by using scanning reader. The linear regression correlation coefficient (R2) was 0.9809. This system shows promise for use in aptamer-conjugated gold nanoparticle probes in paper strip format for in-field detection of marine bioindicating bacteria.",

keywords = "aptamer-based paper strip sensor, ssDNA aptamer, Vibrio fischeri",

author = "Shin, \{Woo Ri\} and Sekhon, \{Simranjeet Singh\} and Rhee, \{Sung Keun\} and Ko, \{Jung Ho\} and Ahn, \{Ji Young\} and Jiho Min and Kim, \{Yang Hoon\}",

note = "Publisher Copyright: Copyright {\textcopyright} 2018 American Chemical Society.",

year = "2018",

month = may,

day = "14",

doi = "10.1021/acscombsci.7b00190",

language = "English",

volume = "20",

pages = "261--268",

journal = "ACS Combinatorial Science",

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AU - Shin, Woo Ri

AU - Sekhon, Simranjeet Singh

AU - Rhee, Sung Keun

AU - Ko, Jung Ho

AU - Ahn, Ji Young

AU - Min, Jiho

AU - Kim, Yang Hoon

PY - 2018/5/14

Y1 - 2018/5/14

N2 - Aptamer-based paper strip sensor for detecting Vibrio fischeri was developed. Our method was based on the aptamer sandwich assay between whole live cells, V. fischeri and DNA aptamer probes. Following 9 rounds of Cell-SELEX and one of the negative-SELEX, V. fischeri Cell Aptamer (VFCA)-02 and -03 were isolated, with the former showing approximately 10-fold greater avidity (in the subnanomolar range) for the target cells when arrayed on a surface. The colorimetric response of a paper sensor based on VFCA-02 was linear in the range of 4 × 101 to 4 × 105 CFU/mL of target cell by using scanning reader. The linear regression correlation coefficient (R2) was 0.9809. This system shows promise for use in aptamer-conjugated gold nanoparticle probes in paper strip format for in-field detection of marine bioindicating bacteria.

AB - Aptamer-based paper strip sensor for detecting Vibrio fischeri was developed. Our method was based on the aptamer sandwich assay between whole live cells, V. fischeri and DNA aptamer probes. Following 9 rounds of Cell-SELEX and one of the negative-SELEX, V. fischeri Cell Aptamer (VFCA)-02 and -03 were isolated, with the former showing approximately 10-fold greater avidity (in the subnanomolar range) for the target cells when arrayed on a surface. The colorimetric response of a paper sensor based on VFCA-02 was linear in the range of 4 × 101 to 4 × 105 CFU/mL of target cell by using scanning reader. The linear regression correlation coefficient (R2) was 0.9809. This system shows promise for use in aptamer-conjugated gold nanoparticle probes in paper strip format for in-field detection of marine bioindicating bacteria.

KW - aptamer-based paper strip sensor

KW - ssDNA aptamer

KW - Vibrio fischeri

UR - https://www.scopus.com/pages/publications/85046997202

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DO - 10.1021/acscombsci.7b00190

M3 - Journal article

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AN - SCOPUS:85046997202

SN - 2156-8952

VL - 20

SP - 261

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JO - ACS Combinatorial Science

JF - ACS Combinatorial Science

IS - 5

ER -

Aptamer-Based Paper Strip Sensor for Detecting Vibrio fischeri

Abstract

Keywords

Quacquarelli Symonds(QS) Subject Topics

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