Abstract
The effectiveness of inexpensive agro-industrial wastes such as sugarcane molasses, paper mill effluent, and dairy effluent for the production of polyhydroxyalkanoates (PHAs) using Bacillus subtilis RS1 was assessed in this study. Laboratory-scale fermentations were carried out with both raw and pre-treated agro-industrial effluents, and maximum PHAs yield was observed in pre-treated effluents. Experimental variables such as pH, inoculum dose, and incubation time were further optimized to maximize PHAs yield, and the optimal conditions were identified as incubation time 48 h; pH 7; and inoculum dose 10% v/v. PHAs yield in pre-treated sugarcane molasses increased to 70.5% under optimal conditions. Fourier transform infrared spectroscopy and gas chromatography–mass spectrometric analysis revealed that the extracted polymer was composed of penta- and hexadecanoic acid methyl esters, a copolymer of PHAs. Results indicated that the pre-treated sugarcane molasses could be used as an inexpensive substrate for the production of PHAs.
| Original language | English |
|---|---|
| Pages (from-to) | 5725-5734 |
| Number of pages | 10 |
| Journal | International Journal of Environmental Science and Technology |
| Volume | 16 |
| Issue number | 10 |
| DOIs | |
| State | Published - 2019.10.1 |
UN SDGs
This output contributes to the following UN Sustainable Development Goals (SDGs)
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SDG 12 Responsible Consumption and Production
Keywords
- Agro-industrial effluents
- Bacillus subtilis RS1
- Fermentation
- Hexadecanoic acid
- Polyhydroxyalkanoates
Quacquarelli Symonds(QS) Subject Topics
- Agriculture & Forestry
- Environmental Sciences
- Engineering - Petroleum
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