Biomolecule-loaded chitosan nanoparticles induce apoptosis and molecular changes in cancer cell line (SiHa)

Anbu Sujima Anbu; Palanivel Velmurugan; Jeong Ho Lee; Byung Taek Oh; Perumal Venkatachalam

doi:10.1016/j.ijbiomac.2016.03.042

Biomolecule-loaded chitosan nanoparticles induce apoptosis and molecular changes in cancer cell line (SiHa)

Anbu Sujima Anbu
, Palanivel Velmurugan
, Jeong Ho Lee
, Byung Taek Oh^*
, Perumal Venkatachalam

^*Corresponding author for this work

Environmental Biotechnology

Periyar University
Jeonbuk National University
Sunchang Research Institute of Health and Longevity

Research output: Contribution to journal › Journal article › peer-review

23 Scopus citations

Abstract

The present study reports on the synthesis of chitosan nanoparticles (CNPs) using methanol extracts of Gymnema sylvestre (GS) leaves and Cinnamomum zeylanicum (CZ) bark. Biomolecule-loaded nanoparticles induced apoptosis in a human cervical cancer (SiHa) cell line, and experiments were carried out to elucidate the underlying molecular mechanisms. FT-IR and XRD showed possible functional groups of the biomolecules and the crystalline nature of CNPs, respectively. Transmission electron microscopy images revealed that synthesized GSCNPs and CZCNPs had a smooth spherical shape with average sizes of about 58-80 and 60-120 nm, respectively. Dynamic light scattering studies indicated that both GSCNPs and CZCNs were structurally stable with homogenous and heterogeneous natures, respectively. Furthermore, synthesized GSCNPs and CZCNPs exhibited dose-dependent cytotoxicity against the SiHa cancer cell line, with inhibitory concentration (IC₅₀) values of 102.17 μg/ml, 87.75 μg/ml, 132.74 μg/ml and 90.35 μg/ml for GS leaf extract, GSCNPs, CZBE and CZCNPs, respectively.

Original language	English
Pages (from-to)	18-26
Number of pages	9
Journal	International Journal of Biological Macromolecules
Volume	88
DOIs	https://doi.org/10.1016/j.ijbiomac.2016.03.042
State	Published - 2016.07.1

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Apoptotic pathways
Characterization
Chitosan
In vitro
Nanoparticles

Quacquarelli Symonds(QS) Subject Topics

Agriculture & Forestry
Materials Science
Biological Sciences

Access to Document

10.1016/j.ijbiomac.2016.03.042

Cite this

@article{6a18abeee88f45a3992d9d81a9b4dc5f,

title = "Biomolecule-loaded chitosan nanoparticles induce apoptosis and molecular changes in cancer cell line (SiHa)",

abstract = "The present study reports on the synthesis of chitosan nanoparticles (CNPs) using methanol extracts of Gymnema sylvestre (GS) leaves and Cinnamomum zeylanicum (CZ) bark. Biomolecule-loaded nanoparticles induced apoptosis in a human cervical cancer (SiHa) cell line, and experiments were carried out to elucidate the underlying molecular mechanisms. FT-IR and XRD showed possible functional groups of the biomolecules and the crystalline nature of CNPs, respectively. Transmission electron microscopy images revealed that synthesized GSCNPs and CZCNPs had a smooth spherical shape with average sizes of about 58-80 and 60-120 nm, respectively. Dynamic light scattering studies indicated that both GSCNPs and CZCNs were structurally stable with homogenous and heterogeneous natures, respectively. Furthermore, synthesized GSCNPs and CZCNPs exhibited dose-dependent cytotoxicity against the SiHa cancer cell line, with inhibitory concentration (IC50) values of 102.17 μg/ml, 87.75 μg/ml, 132.74 μg/ml and 90.35 μg/ml for GS leaf extract, GSCNPs, CZBE and CZCNPs, respectively.",

keywords = "Apoptotic pathways, Characterization, Chitosan, In vitro, Nanoparticles",

author = "\{Sujima Anbu\}, Anbu and Palanivel Velmurugan and Lee, \{Jeong Ho\} and Oh, \{Byung Taek\} and Perumal Venkatachalam",

note = "Publisher Copyright: {\textcopyright} 2016 Elsevier B.V.",

year = "2016",

month = jul,

day = "1",

doi = "10.1016/j.ijbiomac.2016.03.042",

language = "English",

volume = "88",

pages = "18--26",

journal = "International Journal of Biological Macromolecules",

issn = "0141-8130",

}

TY - JOUR

T1 - Biomolecule-loaded chitosan nanoparticles induce apoptosis and molecular changes in cancer cell line (SiHa)

AU - Sujima Anbu, Anbu

AU - Velmurugan, Palanivel

AU - Lee, Jeong Ho

AU - Oh, Byung Taek

AU - Venkatachalam, Perumal

PY - 2016/7/1

Y1 - 2016/7/1

N2 - The present study reports on the synthesis of chitosan nanoparticles (CNPs) using methanol extracts of Gymnema sylvestre (GS) leaves and Cinnamomum zeylanicum (CZ) bark. Biomolecule-loaded nanoparticles induced apoptosis in a human cervical cancer (SiHa) cell line, and experiments were carried out to elucidate the underlying molecular mechanisms. FT-IR and XRD showed possible functional groups of the biomolecules and the crystalline nature of CNPs, respectively. Transmission electron microscopy images revealed that synthesized GSCNPs and CZCNPs had a smooth spherical shape with average sizes of about 58-80 and 60-120 nm, respectively. Dynamic light scattering studies indicated that both GSCNPs and CZCNs were structurally stable with homogenous and heterogeneous natures, respectively. Furthermore, synthesized GSCNPs and CZCNPs exhibited dose-dependent cytotoxicity against the SiHa cancer cell line, with inhibitory concentration (IC50) values of 102.17 μg/ml, 87.75 μg/ml, 132.74 μg/ml and 90.35 μg/ml for GS leaf extract, GSCNPs, CZBE and CZCNPs, respectively.

AB - The present study reports on the synthesis of chitosan nanoparticles (CNPs) using methanol extracts of Gymnema sylvestre (GS) leaves and Cinnamomum zeylanicum (CZ) bark. Biomolecule-loaded nanoparticles induced apoptosis in a human cervical cancer (SiHa) cell line, and experiments were carried out to elucidate the underlying molecular mechanisms. FT-IR and XRD showed possible functional groups of the biomolecules and the crystalline nature of CNPs, respectively. Transmission electron microscopy images revealed that synthesized GSCNPs and CZCNPs had a smooth spherical shape with average sizes of about 58-80 and 60-120 nm, respectively. Dynamic light scattering studies indicated that both GSCNPs and CZCNs were structurally stable with homogenous and heterogeneous natures, respectively. Furthermore, synthesized GSCNPs and CZCNPs exhibited dose-dependent cytotoxicity against the SiHa cancer cell line, with inhibitory concentration (IC50) values of 102.17 μg/ml, 87.75 μg/ml, 132.74 μg/ml and 90.35 μg/ml for GS leaf extract, GSCNPs, CZBE and CZCNPs, respectively.

KW - Apoptotic pathways

KW - Characterization

KW - Chitosan

KW - In vitro

KW - Nanoparticles

UR - https://www.scopus.com/pages/publications/84962052969

U2 - 10.1016/j.ijbiomac.2016.03.042

DO - 10.1016/j.ijbiomac.2016.03.042

M3 - Journal article

C2 - 27016087

AN - SCOPUS:84962052969

SN - 0141-8130

VL - 88

SP - 18

EP - 26

JO - International Journal of Biological Macromolecules

JF - International Journal of Biological Macromolecules

ER -

Biomolecule-loaded chitosan nanoparticles induce apoptosis and molecular changes in cancer cell line (SiHa)

Abstract

UN SDGs

Keywords

Quacquarelli Symonds(QS) Subject Topics

Access to Document

Other files and links

Fingerprint

Cite this