Biophysical interactions between self-sufficient cytochrome P450 from Tepidiphilus thermophilus and ilaprazole

Jaejeong You; Yunha Hwang; Yeon Ju Jeong; Soo Jin Yeom; Chul Ho Yun; Hyun Goo Kang; Seung Jae Lee

doi:10.1039/d4dt03320e

Biophysical interactions between self-sufficient cytochrome P450 from Tepidiphilus thermophilus and ilaprazole

Jaejeong You
, Yunha Hwang
, Yeon Ju Jeong
, Soo Jin Yeom
, Chul Ho Yun
, Hyun Goo Kang^*
, Seung Jae Lee^*

^*Corresponding author for this work

Jeonbuk National University
Chonnam National University

Research output: Contribution to journal › Journal article › peer-review

Abstract

Proton pump inhibitors (PPIs), metabolized by cytochrome P450 (P450) enzymes, are widely used to inhibit gastric acid secretion. This study investigated CYP116B46, a self-sufficient monooxygenase with a reductase domain, to elucidate its interaction with ilaprazole, a PPI. Binding assays and docking simulations indicate that CYP116B46 serves as a suitable model for studying PPI metabolism.

Original language	English
Pages (from-to)	1354-1359
Number of pages	6
Journal	Dalton Transactions
Volume	54
Issue number	4
DOIs	https://doi.org/10.1039/d4dt03320e
State	Published - 2025.01.6

Quacquarelli Symonds(QS) Subject Topics

Chemistry

Access to Document

10.1039/d4dt03320e

Cite this

@article{900d2650346842859d387022a8b074b4,

title = "Biophysical interactions between self-sufficient cytochrome P450 from Tepidiphilus thermophilus and ilaprazole",

abstract = "Proton pump inhibitors (PPIs), metabolized by cytochrome P450 (P450) enzymes, are widely used to inhibit gastric acid secretion. This study investigated CYP116B46, a self-sufficient monooxygenase with a reductase domain, to elucidate its interaction with ilaprazole, a PPI. Binding assays and docking simulations indicate that CYP116B46 serves as a suitable model for studying PPI metabolism.",

author = "Jaejeong You and Yunha Hwang and Jeong, \{Yeon Ju\} and Yeom, \{Soo Jin\} and Yun, \{Chul Ho\} and Kang, \{Hyun Goo\} and Lee, \{Seung Jae\}",

note = "Publisher Copyright: {\textcopyright} 2025 The Royal Society of Chemistry.",

year = "2025",

month = jan,

day = "6",

doi = "10.1039/d4dt03320e",

language = "English",

volume = "54",

pages = "1354--1359",

journal = "Dalton Transactions",

issn = "1477-9226",

number = "4",

}

TY - JOUR

T1 - Biophysical interactions between self-sufficient cytochrome P450 from Tepidiphilus thermophilus and ilaprazole

AU - You, Jaejeong

AU - Hwang, Yunha

AU - Jeong, Yeon Ju

AU - Yeom, Soo Jin

AU - Yun, Chul Ho

AU - Kang, Hyun Goo

AU - Lee, Seung Jae

PY - 2025/1/6

Y1 - 2025/1/6

N2 - Proton pump inhibitors (PPIs), metabolized by cytochrome P450 (P450) enzymes, are widely used to inhibit gastric acid secretion. This study investigated CYP116B46, a self-sufficient monooxygenase with a reductase domain, to elucidate its interaction with ilaprazole, a PPI. Binding assays and docking simulations indicate that CYP116B46 serves as a suitable model for studying PPI metabolism.

AB - Proton pump inhibitors (PPIs), metabolized by cytochrome P450 (P450) enzymes, are widely used to inhibit gastric acid secretion. This study investigated CYP116B46, a self-sufficient monooxygenase with a reductase domain, to elucidate its interaction with ilaprazole, a PPI. Binding assays and docking simulations indicate that CYP116B46 serves as a suitable model for studying PPI metabolism.

UR - https://www.scopus.com/pages/publications/85215602503

U2 - 10.1039/d4dt03320e

DO - 10.1039/d4dt03320e

M3 - Journal article

C2 - 39801326

AN - SCOPUS:85215602503

SN - 1477-9226

VL - 54

SP - 1354

EP - 1359

JO - Dalton Transactions

JF - Dalton Transactions

IS - 4

ER -

Biophysical interactions between self-sufficient cytochrome P450 from Tepidiphilus thermophilus and ilaprazole

Abstract

Quacquarelli Symonds(QS) Subject Topics

Access to Document

Other files and links

Fingerprint

Cite this