Cellulose nanocrystals coated with a tannic acid-Fe³⁺ complex as a significant medium for efficient CH₄ microbial biotransformation

Microbial biotransformation of CH₄ gas has been attractive for the production of energy and high-value chemicals. However, insufficient supply of CH₄ in a culture medium needs to be overcome for the efficient utilization of CH₄. Here, we utilized cellulose nanocrystals coated with a tannic acid-Fe³⁺ complex (TA-Fe³⁺CNCs) as a medium component to enhance the gas-liquid mass-transfer performance. TA-Fe³⁺CNCs were well suspended in water without agglomeration, stabilized gas bubbles without coalescence, and increased the gas solubility by 20 % and the k_La value at a rapid inlet gas flow rate. Remarkably, the cell growth rate of Methylomonas sp. DH-1 as model CH₄-utilizing bacteria improved with TA-Fe³⁺CNC concentration without any cytotoxic or antibacterial properties, resulting in higher metabolite production ability such as methanol, pyruvate, formate, and succinate. These results showed that TA-Fe³⁺CNCs could be utilized as a significant component in the culture medium applicable as a promising nanofluid for efficient CH₄ microbial biotransformation.