Characteristics and Antimicrobial Effects of Novel Burkholderia cepacia No. 15-2 Isolated from Compost

To develop the functional-compost containing antifungal substance by using antagonistic microorganisms, Spinacia oleracea L and Rhizoctonia solani Kuhn 0-28 were used as a model plant and phytopathogen, respectively. Total 80 strains were isolated from the compost of various waste foods mixture processed for a year. Among them, No. 15-2 strain was selected due to its highest antifungal activity against R. solani Kuhn O-28 and was identified phyno- and phylogenotypically as Burkholderia cepacia genomovar V, which is rare probability in pathogen, by 16S rDNA sequencing and specific primer pair PCR method. B. cepacia No. 15-2 preferentially dominated during the compost and its cell numbers were maintained almost × 10¹³ cuf/g for 15 days. The morbidity caused by R. solani Kuhn 0-28 in S. oleracea L cultivation was reduced to 40% by addition of B. cepacia No. 15-2. In conclusion, the antifungal compost using B. cepacia No. 15-2 could be applied to biocontrol of various crops' blights caused by fungal pathogen.