Characterization and structure analysis of PLGA/collagen nanofibrous membranes by electrospinning

Poly(DL-lactide-co-glycolide) (PLGA)/marine collagen nanofibrous membranes (PCNMs) with different weight ratio of PLGA to collagen were fabricated by an electrospinning method. The morphology and diameter distribution of electrospun nanofibers were investigated by scanning electron microscopy (SEM). SEM images showed that the morphology and diameter distribution of the nanofibers were mainly affected by the concentration of the solution, weight ratio of the PLGA/collagen, and applied electric voltage, respectively. To investigate the cell responses, MG-63 cells isolated from a human mandible were cultured onto various substrates and their adhesion, spreading, and proliferation were examined. Immunofluorescent staining of MG-63 cultured on the PCNMs demonstrated homogeneous localization of F-Actin and vinculin in their cytoplasms. However, no mature adhesive structures were observed in the cell cultured on other substrates. Also, the cells seeded on the materials proliferated. These results suggest that PCNMs strongly support the attachment and growth of osteosarcoma cells.