Characterization of Platelet-Rich Plasma/Gellan Gum Hydrogel Composite for Biological Performance to Induce Chondrogenesis from Adipose-Derived Stem Cells

In this experiment, hydrogel composite was developed with gellan gum (GG) and platelet-rich plasma (PRP) to induce chondrogenesis of adipose-derived stem cells (ADSCs) in the hydrated environment. FTIR, SEM, and compression test were carried out to characterize the fabricated material. Biocompatibility was analyzed by MTT assay. The morphology of the encapsulated ADSCs was observed under SEM. Chondrogenesis was examined by PCR. The chemical and morphological analysis displayed the appearance of PRP in the matrix. The mechanical property of the hydrogel decreased as the PRP was loaded but viability and chondrogenesis were significantly increased with the proper amount of the PRP. Overall, the composite of the appropriate incorporation of PRP in the GG can enhance the microenvironment for chondrogenesis and expected to have great potential in tissue engineering.[Figure not available: see fulltext.]