Characterization, real-time quantification and in silico modeling of arsenate reductase (arsC) genes in arsenic-resistant Herbaspirillum sp. GW103

Muthusamy Govarthanan; Sang Myeong Lee; Seralathan Kamala-Kannan; Byung Taek Oh

doi:10.1016/j.resmic.2015.02.007

Characterization, real-time quantification and in silico modeling of arsenate reductase (arsC) genes in arsenic-resistant Herbaspirillum sp. GW103

Muthusamy Govarthanan
, Sang Myeong Lee
, Seralathan Kamala-Kannan^*
, Byung Taek Oh

^*Corresponding author for this work

Environmental Biotechnology

Jeonbuk National University

Research output: Contribution to journal › Journal article › peer-review

26 Scopus citations

Abstract

This study investigated the mechanism of arsenic resistance in the diazotrophic bacterium Herbaspirillum sp. GW103 isolated from rhizosphere soil of Phragmites austrails. The isolate Herbaspirillum sp. GW103 exhibited maximum tolerance to arsenic (550mg/L). Four different arsenate reductase (. arsC) genes (. arsC1, arsC2, arsC3 and arsC4) were located in the genome of the isolate Herbaspirillum sp. GW103. The expression pattern of the arsC1 differed from other genes. All four types of arsC genes had different protein secondary structures and stereochemical properties. Molecular modeling and structural analysis of arsC genes revealed close structural homology with arsC family proteins from Escherichia coli (PDB ID: 1I9D) and Pseudomonas aeruginosa (PDB ID: 1RW1).

Original language	English
Pages (from-to)	196-204
Number of pages	9
Journal	Research in Microbiology
Volume	166
Issue number	3
DOIs	https://doi.org/10.1016/j.resmic.2015.02.007
State	Published - 2015.04.1

Keywords

Arsenate reductase
Heavy metals
Herbaspirillum sp.
Real-time PCR

Quacquarelli Symonds(QS) Subject Topics

Biological Sciences

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10.1016/j.resmic.2015.02.007

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@article{9416da3d8fe848aea71d0f29cd68d9c3,

title = "Characterization, real-time quantification and in silico modeling of arsenate reductase (arsC) genes in arsenic-resistant Herbaspirillum sp. GW103",

abstract = "This study investigated the mechanism of arsenic resistance in the diazotrophic bacterium Herbaspirillum sp. GW103 isolated from rhizosphere soil of Phragmites austrails. The isolate Herbaspirillum sp. GW103 exhibited maximum tolerance to arsenic (550mg/L). Four different arsenate reductase (. arsC) genes (. arsC1, arsC2, arsC3 and arsC4) were located in the genome of the isolate Herbaspirillum sp. GW103. The expression pattern of the arsC1 differed from other genes. All four types of arsC genes had different protein secondary structures and stereochemical properties. Molecular modeling and structural analysis of arsC genes revealed close structural homology with arsC family proteins from Escherichia coli (PDB ID: 1I9D) and Pseudomonas aeruginosa (PDB ID: 1RW1).",

keywords = "Arsenate reductase, Heavy metals, Herbaspirillum sp., Real-time PCR",

author = "Muthusamy Govarthanan and Lee, \{Sang Myeong\} and Seralathan Kamala-Kannan and Oh, \{Byung Taek\}",

note = "Publisher Copyright: {\textcopyright} 2015 Institut Pasteur.",

year = "2015",

month = apr,

day = "1",

doi = "10.1016/j.resmic.2015.02.007",

language = "English",

volume = "166",

pages = "196--204",

journal = "Research in Microbiology",

issn = "0923-2508",

number = "3",

}

Characterization, real-time quantification and in silico modeling of arsenate reductase (arsC) genes in arsenic-resistant Herbaspirillum sp. GW103. / Govarthanan, Muthusamy; Lee, Sang Myeong; Kamala-Kannan, Seralathan et al.
In: Research in Microbiology, Vol. 166, No. 3, 01.04.2015, p. 196-204.

Research output: Contribution to journal › Journal article › peer-review

TY - JOUR

T1 - Characterization, real-time quantification and in silico modeling of arsenate reductase (arsC) genes in arsenic-resistant Herbaspirillum sp. GW103

AU - Govarthanan, Muthusamy

AU - Lee, Sang Myeong

AU - Kamala-Kannan, Seralathan

AU - Oh, Byung Taek

PY - 2015/4/1

Y1 - 2015/4/1

N2 - This study investigated the mechanism of arsenic resistance in the diazotrophic bacterium Herbaspirillum sp. GW103 isolated from rhizosphere soil of Phragmites austrails. The isolate Herbaspirillum sp. GW103 exhibited maximum tolerance to arsenic (550mg/L). Four different arsenate reductase (. arsC) genes (. arsC1, arsC2, arsC3 and arsC4) were located in the genome of the isolate Herbaspirillum sp. GW103. The expression pattern of the arsC1 differed from other genes. All four types of arsC genes had different protein secondary structures and stereochemical properties. Molecular modeling and structural analysis of arsC genes revealed close structural homology with arsC family proteins from Escherichia coli (PDB ID: 1I9D) and Pseudomonas aeruginosa (PDB ID: 1RW1).

AB - This study investigated the mechanism of arsenic resistance in the diazotrophic bacterium Herbaspirillum sp. GW103 isolated from rhizosphere soil of Phragmites austrails. The isolate Herbaspirillum sp. GW103 exhibited maximum tolerance to arsenic (550mg/L). Four different arsenate reductase (. arsC) genes (. arsC1, arsC2, arsC3 and arsC4) were located in the genome of the isolate Herbaspirillum sp. GW103. The expression pattern of the arsC1 differed from other genes. All four types of arsC genes had different protein secondary structures and stereochemical properties. Molecular modeling and structural analysis of arsC genes revealed close structural homology with arsC family proteins from Escherichia coli (PDB ID: 1I9D) and Pseudomonas aeruginosa (PDB ID: 1RW1).

KW - Arsenate reductase

KW - Heavy metals

KW - Herbaspirillum sp.

KW - Real-time PCR

UR - https://www.scopus.com/pages/publications/84926420511

U2 - 10.1016/j.resmic.2015.02.007

DO - 10.1016/j.resmic.2015.02.007

M3 - Journal article

C2 - 25744778

AN - SCOPUS:84926420511

SN - 0923-2508

VL - 166

SP - 196

EP - 204

JO - Research in Microbiology

JF - Research in Microbiology

IS - 3

ER -

Characterization, real-time quantification and in silico modeling of arsenate reductase (arsC) genes in arsenic-resistant Herbaspirillum sp. GW103

Abstract

Keywords

Quacquarelli Symonds(QS) Subject Topics

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