Chondrogenesis of rat bone marrow stromal cells in transforming growth factor-β1 loaded alginate bead in in vivo

Soon Hee Kim; Ji Wook Jang; Su Hyun Jung; Jin Hee Choi; Hyun Jung Ha; John M. Rhee; Young Sun Kang; Gilson Khang

Chondrogenesis of rat bone marrow stromal cells in transforming growth factor-β1 loaded alginate bead in in vivo

Soon Hee Kim
, Ji Wook Jang
, Su Hyun Jung
, Jin Hee Choi
, Hyun Jung Ha
, John M. Rhee
, Young Sun Kang
, Gilson Khang

Department of Polymer -Nano Science &Technology

Jeonbuk National University
Konkuk University

Research output: Contribution to journal › Journal article › peer-review

5 Scopus citations

Abstract

This study aimed to evaluate the chondrogenic potential of alginate gel loaded transforming growth factor beta 1(TGF-β1) and bone marrow derived stem cells(BMSCs). We, based on previous in vitro experiment, encapsulated cell and TGF-β1 into alginate bead and transplanted on the dorsum of nude mouse subcutaneously. The implantations were harvested and evaluated by histology through H&E, Alcian Blue, Safranin-O and immunohis-tochemistry(IHC) and immunofluorescence(IF) staining. These results presented that the alginate bead incorporated with TGF-β1 and BMSC is promising for cartilage tissue engineering application and that specially is more helpful to chondrogenesis in a limited area than alginate with nothing or the alternative TGF-β1 or BMSC.

Original language	English
Pages (from-to)	650-659
Number of pages	10
Journal	Tissue Engineering and Regenerative Medicine
Volume	5
Issue number	4-6
State	Published - 2008.12

Keywords

Alginate bead
BMSC
Chondrogenesis
In vivo
TGF-β1

Quacquarelli Symonds(QS) Subject Topics

Medicine
Biological Sciences

Cite this

@article{de14ff7014074d21beae409f710adac3,

title = "Chondrogenesis of rat bone marrow stromal cells in transforming growth factor-β1 loaded alginate bead in in vivo",

abstract = "This study aimed to evaluate the chondrogenic potential of alginate gel loaded transforming growth factor beta 1(TGF-β1) and bone marrow derived stem cells(BMSCs). We, based on previous in vitro experiment, encapsulated cell and TGF-β1 into alginate bead and transplanted on the dorsum of nude mouse subcutaneously. The implantations were harvested and evaluated by histology through H\&E, Alcian Blue, Safranin-O and immunohis-tochemistry(IHC) and immunofluorescence(IF) staining. These results presented that the alginate bead incorporated with TGF-β1 and BMSC is promising for cartilage tissue engineering application and that specially is more helpful to chondrogenesis in a limited area than alginate with nothing or the alternative TGF-β1 or BMSC.",

keywords = "Alginate bead, BMSC, Chondrogenesis, In vivo, TGF-β1",

author = "Kim, \{Soon Hee\} and Jang, \{Ji Wook\} and Jung, \{Su Hyun\} and Choi, \{Jin Hee\} and Ha, \{Hyun Jung\} and Rhee, \{John M.\} and Kang, \{Young Sun\} and Gilson Khang",

year = "2008",

month = dec,

language = "English",

volume = "5",

pages = "650--659",

journal = "Tissue Engineering and Regenerative Medicine",

issn = "1738-2696",

number = "4-6",

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TY - JOUR

T1 - Chondrogenesis of rat bone marrow stromal cells in transforming growth factor-β1 loaded alginate bead in in vivo

AU - Kim, Soon Hee

AU - Jang, Ji Wook

AU - Jung, Su Hyun

AU - Choi, Jin Hee

AU - Ha, Hyun Jung

AU - Rhee, John M.

AU - Kang, Young Sun

AU - Khang, Gilson

PY - 2008/12

Y1 - 2008/12

N2 - This study aimed to evaluate the chondrogenic potential of alginate gel loaded transforming growth factor beta 1(TGF-β1) and bone marrow derived stem cells(BMSCs). We, based on previous in vitro experiment, encapsulated cell and TGF-β1 into alginate bead and transplanted on the dorsum of nude mouse subcutaneously. The implantations were harvested and evaluated by histology through H&E, Alcian Blue, Safranin-O and immunohis-tochemistry(IHC) and immunofluorescence(IF) staining. These results presented that the alginate bead incorporated with TGF-β1 and BMSC is promising for cartilage tissue engineering application and that specially is more helpful to chondrogenesis in a limited area than alginate with nothing or the alternative TGF-β1 or BMSC.

AB - This study aimed to evaluate the chondrogenic potential of alginate gel loaded transforming growth factor beta 1(TGF-β1) and bone marrow derived stem cells(BMSCs). We, based on previous in vitro experiment, encapsulated cell and TGF-β1 into alginate bead and transplanted on the dorsum of nude mouse subcutaneously. The implantations were harvested and evaluated by histology through H&E, Alcian Blue, Safranin-O and immunohis-tochemistry(IHC) and immunofluorescence(IF) staining. These results presented that the alginate bead incorporated with TGF-β1 and BMSC is promising for cartilage tissue engineering application and that specially is more helpful to chondrogenesis in a limited area than alginate with nothing or the alternative TGF-β1 or BMSC.

KW - Alginate bead

KW - BMSC

KW - Chondrogenesis

KW - In vivo

KW - TGF-β1

UR - https://www.scopus.com/pages/publications/79957531993

M3 - Journal article

AN - SCOPUS:79957531993

SN - 1738-2696

VL - 5

SP - 650

EP - 659

JO - Tissue Engineering and Regenerative Medicine

JF - Tissue Engineering and Regenerative Medicine

IS - 4-6

ER -

Chondrogenesis of rat bone marrow stromal cells in transforming growth factor-β1 loaded alginate bead in in vivo

Abstract

Keywords

Quacquarelli Symonds(QS) Subject Topics

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