Abstract
The cDNA of acetyl xylan esterase 2 (PcAxe2) gene containing a carbohydrate binding module (CBM) sequence from Phanerochaete chrysosporium was cloned and expressed in Pichia pastoris. The recombinant PcAxe2 protein (rPcAxe2) was efficiently produced, reaching a maximum of 1058 U l-1 after 6 days of cultivation. Molecular mass of the rPcAxe2 on SDS-PAGE was approximately 63 kDa under hyperglycosylation. Optimal activity of the purified rPcAxe2 enzyme was observed at pH and temperature of 7.0 and 30-35°C, respectively. In addition to acetyl xylan esterase activity, rPcAxe2 also exhibited a xylanase activity at an optimum pH and temperature of 5.0 and 80°C, respectively. The synergistic action of rPcAxe2 with rPcXynC on birchwood xylan, beechwood xylan and wheat arabinoxylan enhanced the total reducing soluble sugar.
| Original language | English |
|---|---|
| Pages (from-to) | 507-513 |
| Number of pages | 7 |
| Journal | Journal of Bioscience and Bioengineering |
| Volume | 115 |
| Issue number | 5 |
| DOIs | |
| State | Published - 2013.05 |
Keywords
- Acetyl xylan esterase
- Phanerochaete chrysosporium
- Pichia pastoris
- Synergisms
- Xylanase
Quacquarelli Symonds(QS) Subject Topics
- Engineering - Chemical
- Biological Sciences
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