Cobalt chloride-induced downregulation of puromycin-sensitive Aminopeptidase suppresses the migration and invasion of PC-3 cells

Cobalt chloride (CoCl₂) treatment of cells in vitro has been shown to induce cellular changes that are similar those seen following hypoxia. To identify genes that differentially expressed in response to treatment with CoCl₂, we compared the mRNA expression profiles of PC-cells that were treated with CoCl₂ with those of untreated PC-3 cells, using specific arbitrary primers and two anchored oligo(dT) primers provided in the ACP-based GeneFishing kits. The results of this study demonstrated that the puromycin-sensitive aminopeptidase (PSA) gene was downregulated in PC-3 cells that were treated with CoCl₂. This downregulation of PSA expression, in turn, suppressed the proliferation, migration, and invasion PC-3 cells, as well as the secretion and expression matrix metalloproteinase-9 (MMP-9).