Abstract
A protein chip based on surface plasmon resonance imaging (SPRI) was developed for detecting fish iridovirus antibody using a recombinant 50-kDa fragment of major capsid protein (MCP) as an antigen. The diagnostic potential of SPRI for measuring antibodies to the iridovirus MCP was compared with that of a conventional enzyme-linked immunosorbent assay (ELISA) using 40 juvenile rock bream (Oplegnathus fasciatus) serum samples in a nursery. There was a strong positive correlation between the SPRI and ELISA (n = 40, r = 0.939, P < 0.01). Therefore, this recombinant 50-kDa MCP can be used as an antigen for serological studies, and the SPRI, which is a label-free and high-throughput method, is potentially a valuable tool in the serodiagnosis of an iridoviral infection.
| Original language | English |
|---|---|
| Pages (from-to) | 414-416 |
| Number of pages | 3 |
| Journal | Journal of Veterinary Diagnostic Investigation |
| Volume | 19 |
| Issue number | 4 |
| DOIs | |
| State | Published - 2007.07 |
Keywords
- ELISA
- Iridovirus
- Serodiagnosis
- Surface plasmon resonance imaging (SPRI)
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