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Construction and characterisation of an antifungal recombinant Bacillus thuringiensis with an expanded host spectrum

  • Qin Liu
  • , Jong Yul Roh
  • , Yong Wang
  • , Jae Young Choi
  • , Xue Ying Tao
  • , Jae Su Kim
  • , Yeon Ho Je
  • Seoul National University
  • Korea National Institute of Health
  • Lanzhou University

Research output: Contribution to journalJournal articlepeer-review

Abstract

A novel antifungal Bacillus thuringiensis strain 19-22, ssp. kurstaki (H3a3b3c), was characterised. This strain included cry1Aa, cry1Ab, cry1Ac, and cry1D, which have high insecticidal activities against lepidopteran larvae other than Spodoptera exigua. To expand the host spectrum, a cry1E gene whose product is active against S. exigua was introduced into the isolate. The transformant successfully expressed the Cry1E protein without any loss of its original antifungal activities. These results indicate that this recombinant strain exhibits dual activities and may be used as an integrated control agent to control plant diseases and insect pests.

Original languageEnglish
Pages (from-to)874-877
Number of pages4
JournalJournal of Microbiology
Volume50
Issue number5
DOIs
StatePublished - 2012.10

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

  1. SDG 2 - Zero Hunger
    SDG 2 Zero Hunger

Keywords

  • antifungal activity
  • Bacillus thuringiensis
  • cry1E
  • insecticidal activity

Quacquarelli Symonds(QS) Subject Topics

  • Biological Sciences

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