Deletion of p22^phox-dependent oxidative stress in the hypothalamus protects against obesity by modulating β₃-adrenergic mechanisms

A role for oxidative stress in the brain has been suggested in the pathogenesis of diet-induced obesity (DIO), although the underlying neural regions and mechanisms remain incompletely defined. We tested the hypothesis that NADPH oxidase–dependent oxidative stress in the paraventricular nucleus (PVN), a hypothalamic energy homeostasis center, contributes to the development of DIO. Cre/LoxP technology was coupled with selective PVN adenoviral microinjection to ablate p22^phox, the obligatory subunit for NADPH oxidase activity, in mice harboring a conditional p22^phox allele. Selective deletion of p22^phox in the PVN protected mice from high-fat DIO independent of changes in food intake or locomotor activity. This was accompanied by β₃-adrenoceptor–dependent increases in energy expenditure, elevations in brown adipose tissue thermogenesis, and browning of white adipose tissue. These data reveal a potentially novel role for brain oxidative stress in the development of DIO by modulating β₃-adrenoceptor mechanisms and point to the PVN as an underlying neural site.