Development and characterization of a catalytically inactive cysteine protease domain of RtxA1/MARTXVv as a potential vaccine for Vibrio vulnificus

Tae Hee Lee; Kyung Min Chung

doi:10.1111/1348-0421.12284

Development and characterization of a catalytically inactive cysteine protease domain of RtxA1/MARTX_Vv as a potential vaccine for Vibrio vulnificus

Tae Hee Lee
, Kyung Min Chung^*

^*Corresponding author for this work

Department of Medicine

Jeonbuk National University

Research output: Contribution to journal › Journal article › peer-review

2 Scopus citations

Abstract

Recent studies have defined several virulence factors as vaccine candidates against Vibrio vulnificus. However, most of these factors have the potential to cause pathogenic effects in the vaccinees or induce incomplete protection. To overcome these drawbacks, a catalytically inactive form, CPD_Vv(C3725S), of the well-conserved cysteine protease domain (CPD) of V. vulnificus multifunctional autoprocessing repeats-in-toxin (MARTX_Vv/RtxA1) was recombinantly generated and characterized. Notably, active and passive immunization with CPD_Vv(C3725S) conferred protective immunity against V. vulnificus strains. These results may provide a novel framework for developing safe and efficient subunit vaccines and/or therapeutics against V. vulnificus that target the CPD of MARTX toxins.

Original language	English
Pages (from-to)	555-561
Number of pages	7
Journal	Microbiology and Immunology
Volume	59
Issue number	9
DOIs	https://doi.org/10.1111/1348-0421.12284
State	Published - 2015.09.1

Keywords

Cysteine protease domain
Protective immunity
RtxA1/MARTX
Vibrio vulnificus

Quacquarelli Symonds(QS) Subject Topics

Biological Sciences

Access to Document

10.1111/1348-0421.12284

Cite this

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title = "Development and characterization of a catalytically inactive cysteine protease domain of RtxA1/MARTXVv as a potential vaccine for Vibrio vulnificus",

abstract = "Recent studies have defined several virulence factors as vaccine candidates against Vibrio vulnificus. However, most of these factors have the potential to cause pathogenic effects in the vaccinees or induce incomplete protection. To overcome these drawbacks, a catalytically inactive form, CPDVv(C3725S), of the well-conserved cysteine protease domain (CPD) of V. vulnificus multifunctional autoprocessing repeats-in-toxin (MARTXVv/RtxA1) was recombinantly generated and characterized. Notably, active and passive immunization with CPDVv(C3725S) conferred protective immunity against V. vulnificus strains. These results may provide a novel framework for developing safe and efficient subunit vaccines and/or therapeutics against V. vulnificus that target the CPD of MARTX toxins.",

keywords = "Cysteine protease domain, Protective immunity, RtxA1/MARTX, Vibrio vulnificus",

author = "Lee, \{Tae Hee\} and Chung, \{Kyung Min\}",

note = "Publisher Copyright: {\textcopyright} 2015 The Societies and Wiley Publishing Asia Pty Ltd.",

year = "2015",

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T1 - Development and characterization of a catalytically inactive cysteine protease domain of RtxA1/MARTXVv as a potential vaccine for Vibrio vulnificus

AU - Lee, Tae Hee

AU - Chung, Kyung Min

PY - 2015/9/1

Y1 - 2015/9/1

N2 - Recent studies have defined several virulence factors as vaccine candidates against Vibrio vulnificus. However, most of these factors have the potential to cause pathogenic effects in the vaccinees or induce incomplete protection. To overcome these drawbacks, a catalytically inactive form, CPDVv(C3725S), of the well-conserved cysteine protease domain (CPD) of V. vulnificus multifunctional autoprocessing repeats-in-toxin (MARTXVv/RtxA1) was recombinantly generated and characterized. Notably, active and passive immunization with CPDVv(C3725S) conferred protective immunity against V. vulnificus strains. These results may provide a novel framework for developing safe and efficient subunit vaccines and/or therapeutics against V. vulnificus that target the CPD of MARTX toxins.

AB - Recent studies have defined several virulence factors as vaccine candidates against Vibrio vulnificus. However, most of these factors have the potential to cause pathogenic effects in the vaccinees or induce incomplete protection. To overcome these drawbacks, a catalytically inactive form, CPDVv(C3725S), of the well-conserved cysteine protease domain (CPD) of V. vulnificus multifunctional autoprocessing repeats-in-toxin (MARTXVv/RtxA1) was recombinantly generated and characterized. Notably, active and passive immunization with CPDVv(C3725S) conferred protective immunity against V. vulnificus strains. These results may provide a novel framework for developing safe and efficient subunit vaccines and/or therapeutics against V. vulnificus that target the CPD of MARTX toxins.

KW - Cysteine protease domain

KW - Protective immunity

KW - RtxA1/MARTX

KW - Vibrio vulnificus

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