Development of a point-of-care test system for the analysis of total cholesterol in serum sample

This study has developed a fluorescence Chromatographic assay for the quantification of total cholesterol in serum. The new assay system consists of a Chromatographic strip in a cartridge, enzyme containing cholesterol oxidase (CO), cholesterol esterase (CE), horseradish peroxidase (HRP) and color developer 3-amino-9-ethyl carbazole (AEC), and a laser fluorescence scanner. The correlation coefficient R between cholesterol concentration and the relative fluorescence units was 0.979 within 10 % of CV in the new assay, showing a reliable linearity through the tested range of cholesterol. Recovery test and comparability with a Hitachi 747 instrument showed 106.5 - 94.0 % and r = 0.895, respectively. The new assay system for total cholesterol was developed as a POCT(Point-of-Care Test)plat form for on-site diagnostics since it is fast(8 min) and uses a small volume of sample(5 μL), and it may apply for on-site diagnostics to replace expensive automated biochemical analyzer.