Development of protein chip based on surface plasmon resonance for the detection of actinobacillus pleuropneumoniae antibody

Tae Jung Kim; Ho Seong Cho; Jae Il Lee; Nam Yong Park

doi:10.1111/j.1745-4581.2007.00065.x

Development of protein chip based on surface plasmon resonance for the detection of actinobacillus pleuropneumoniae antibody

Tae Jung Kim
, Ho Seong Cho
, Jae Il Lee
, Nam Yong Park^*

^*Corresponding author for this work

Department of Veterinary Medicine

Biotherapy Human Resource Center
Chonnam National University

Research output: Contribution to journal › Journal article › peer-review

2 Scopus citations

Abstract

A protein chip based on surface plasmon resonance (SPR) was developed for measuring the Actinobacillus pleuropneumoniae (APP) antibody (Ab) titers using recombinant transferrin-binding protein B (Tbp B) as an antigen. The diagnostic potential of this SPR assay for detecting the Ab titers to APP Tbp B was compared with that of enzyme-linked immunosorbent assay (ELISA) using 70 serum samples obtained from six pig farms. The SPR assay was found to be highly specific and sensitive. Moreover, there was a strong positive correlation between the SPR and ELISA titers (n = 70, r = 0.858, P < 0.01). In conclusion, the SPR, which is a label-free method, is expected to be a valuable and reproducible tool in the serodiagnosis of an APP infection.

Original language	English
Pages (from-to)	206-215
Number of pages	10
Journal	Journal of Rapid Methods and Automation in Microbiology
Volume	15
Issue number	2
DOIs	https://doi.org/10.1111/j.1745-4581.2007.00065.x
State	Published - 2007.06

Access to Document

10.1111/j.1745-4581.2007.00065.x

Cite this

@article{c07eb708776c46c49ecced73e2170178,

title = "Development of protein chip based on surface plasmon resonance for the detection of actinobacillus pleuropneumoniae antibody",

abstract = "A protein chip based on surface plasmon resonance (SPR) was developed for measuring the Actinobacillus pleuropneumoniae (APP) antibody (Ab) titers using recombinant transferrin-binding protein B (Tbp B) as an antigen. The diagnostic potential of this SPR assay for detecting the Ab titers to APP Tbp B was compared with that of enzyme-linked immunosorbent assay (ELISA) using 70 serum samples obtained from six pig farms. The SPR assay was found to be highly specific and sensitive. Moreover, there was a strong positive correlation between the SPR and ELISA titers (n = 70, r = 0.858, P < 0.01). In conclusion, the SPR, which is a label-free method, is expected to be a valuable and reproducible tool in the serodiagnosis of an APP infection.",

author = "Kim, \{Tae Jung\} and Cho, \{Ho Seong\} and Lee, \{Jae Il\} and Park, \{Nam Yong\}",

year = "2007",

month = jun,

doi = "10.1111/j.1745-4581.2007.00065.x",

language = "English",

volume = "15",

pages = "206--215",

journal = "Journal of Rapid Methods and Automation in Microbiology",

issn = "1060-3999",

number = "2",

}

TY - JOUR

T1 - Development of protein chip based on surface plasmon resonance for the detection of actinobacillus pleuropneumoniae antibody

AU - Kim, Tae Jung

AU - Cho, Ho Seong

AU - Lee, Jae Il

AU - Park, Nam Yong

PY - 2007/6

Y1 - 2007/6

N2 - A protein chip based on surface plasmon resonance (SPR) was developed for measuring the Actinobacillus pleuropneumoniae (APP) antibody (Ab) titers using recombinant transferrin-binding protein B (Tbp B) as an antigen. The diagnostic potential of this SPR assay for detecting the Ab titers to APP Tbp B was compared with that of enzyme-linked immunosorbent assay (ELISA) using 70 serum samples obtained from six pig farms. The SPR assay was found to be highly specific and sensitive. Moreover, there was a strong positive correlation between the SPR and ELISA titers (n = 70, r = 0.858, P < 0.01). In conclusion, the SPR, which is a label-free method, is expected to be a valuable and reproducible tool in the serodiagnosis of an APP infection.

AB - A protein chip based on surface plasmon resonance (SPR) was developed for measuring the Actinobacillus pleuropneumoniae (APP) antibody (Ab) titers using recombinant transferrin-binding protein B (Tbp B) as an antigen. The diagnostic potential of this SPR assay for detecting the Ab titers to APP Tbp B was compared with that of enzyme-linked immunosorbent assay (ELISA) using 70 serum samples obtained from six pig farms. The SPR assay was found to be highly specific and sensitive. Moreover, there was a strong positive correlation between the SPR and ELISA titers (n = 70, r = 0.858, P < 0.01). In conclusion, the SPR, which is a label-free method, is expected to be a valuable and reproducible tool in the serodiagnosis of an APP infection.

UR - https://www.scopus.com/pages/publications/34249693571

U2 - 10.1111/j.1745-4581.2007.00065.x

DO - 10.1111/j.1745-4581.2007.00065.x

M3 - Journal article

AN - SCOPUS:34249693571

SN - 1060-3999

VL - 15

SP - 206

EP - 215

JO - Journal of Rapid Methods and Automation in Microbiology

JF - Journal of Rapid Methods and Automation in Microbiology

IS - 2

ER -

Development of protein chip based on surface plasmon resonance for the detection of actinobacillus pleuropneumoniae antibody

Abstract

Access to Document

Other files and links

Fingerprint

Cite this