Diclofenac modified the root system architecture of Arabidopsis via interfering with the hormonal activities of auxin

Diclofenac, a pharmaceutical and personal care product, is accumulating in various environmental matrices worldwide. Increased irrigation has facilitated an influx of environmental diclofenac into agricultural products, which potentially threatens non-target living organisms. In this study, we demonstrated that diclofenac modified the growth and root developmental processes of plants by disturbing the activity of auxin, a group of major phytohormones. Exogenous diclofenac treatment retarded growth and induced oxidative stress in young seedlings of Arabidopsis thaliana. In the developmental perspective, diclofenac altered the root system architecture, which was also similarly observed under exogenous IAA (a natural form of phytoalexins) treatment. The effects of diclofenac on the root development of A. thaliana were mediated through canonical auxin signaling pathways. However, when diclofenac and IAA were treated in combination, diclofenac suppressed the activity of IAA in root system architecture. At the molecular level, diclofenac significantly inhibited the activity of IAA upregulating the expression of early auxin-responsive marker genes. In conclusion, diclofenac modified the root development of A. thaliana via interfering with the activities of natural auxin. These results indicate that diclofenac could potentially act as an environmental contaminant disturbing the natural developmental processes of plants.