Different expression patterns of lysosomal proteins exposed to melanin in HeLa cells

Dong Jun Park; Simranjeet Singh Sekhon; Ji Young Ahn; Hobaek Yoon; Lyon Lee; Jung Ho Ko; Yang Hoon Kim; Jiho Min

doi:10.1007/s13530-015-0248-7

Different expression patterns of lysosomal proteins exposed to melanin in HeLa cells

Dong Jun Park
, Simranjeet Singh Sekhon
, Ji Young Ahn
, Hobaek Yoon
, Lyon Lee
, Jung Ho Ko
, Yang Hoon Kim
, Jiho Min^*

^*Corresponding author for this work

Division of Chemical Engineering

Chungbuk National University
Rural Development Administration
Western University of Health Sciences

Research output: Contribution to journal › Journal article › peer-review

1 Scopus citations

Abstract

As a high quantity of melanin can lead to hyperpigmentation or skin cancer in humans and it can also activate lysosomal enzymes so in the present study the changes in lysosomal enzymes upon exposure of HeLa cells to melanin has been studied. The HeLa cells were exposed to 100 ppm melanin for 24 hours and lysosomal enzymes were extracted. The lysosomal enzymes were identified through twodimensional polyacrylamide gel electrophoresis (2DE) after exposure of HeLa cells to melanin. The results showed 12 up and 3down regulation spots in relation to melanin exposure. It has been observed that lysosomal proteins are related to melanin to decrease the color and quantity through cellular activation of lysosomes.

Original language	English
Pages (from-to)	272-276
Number of pages	5
Journal	Toxicology and Environmental Health Sciences
Volume	7
Issue number	5
DOIs	https://doi.org/10.1007/s13530-015-0248-7
State	Published - 2015.12.1

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Lysosomal proteins
Melanin
Two-dimensional gel electrophoresis (2-DE)

Quacquarelli Symonds(QS) Subject Topics

Environmental Sciences
Pharmacy & Pharmacology

Access to Document

10.1007/s13530-015-0248-7

Cite this

@article{cdce13ca6e5f4b63b811c1867393b826,

title = "Different expression patterns of lysosomal proteins exposed to melanin in HeLa cells",

abstract = "As a high quantity of melanin can lead to hyperpigmentation or skin cancer in humans and it can also activate lysosomal enzymes so in the present study the changes in lysosomal enzymes upon exposure of HeLa cells to melanin has been studied. The HeLa cells were exposed to 100 ppm melanin for 24 hours and lysosomal enzymes were extracted. The lysosomal enzymes were identified through twodimensional polyacrylamide gel electrophoresis (2DE) after exposure of HeLa cells to melanin. The results showed 12 up and 3down regulation spots in relation to melanin exposure. It has been observed that lysosomal proteins are related to melanin to decrease the color and quantity through cellular activation of lysosomes.",

keywords = "Lysosomal proteins, Melanin, Two-dimensional gel electrophoresis (2-DE)",

author = "Park, \{Dong Jun\} and Sekhon, \{Simranjeet Singh\} and Ahn, \{Ji Young\} and Hobaek Yoon and Lyon Lee and Ko, \{Jung Ho\} and Kim, \{Yang Hoon\} and Jiho Min",

note = "Publisher Copyright: {\textcopyright} 2015, Korean Society of Environmental Risk Assessment and Health Science and Springer Science+Business Media Dordrecht.",

year = "2015",

month = dec,

day = "1",

doi = "10.1007/s13530-015-0248-7",

language = "English",

volume = "7",

pages = "272--276",

journal = "Toxicology and Environmental Health Sciences",

issn = "2005-9752",

number = "5",

}

TY - JOUR

T1 - Different expression patterns of lysosomal proteins exposed to melanin in HeLa cells

AU - Park, Dong Jun

AU - Sekhon, Simranjeet Singh

AU - Ahn, Ji Young

AU - Yoon, Hobaek

AU - Lee, Lyon

AU - Ko, Jung Ho

AU - Kim, Yang Hoon

AU - Min, Jiho

PY - 2015/12/1

Y1 - 2015/12/1

N2 - As a high quantity of melanin can lead to hyperpigmentation or skin cancer in humans and it can also activate lysosomal enzymes so in the present study the changes in lysosomal enzymes upon exposure of HeLa cells to melanin has been studied. The HeLa cells were exposed to 100 ppm melanin for 24 hours and lysosomal enzymes were extracted. The lysosomal enzymes were identified through twodimensional polyacrylamide gel electrophoresis (2DE) after exposure of HeLa cells to melanin. The results showed 12 up and 3down regulation spots in relation to melanin exposure. It has been observed that lysosomal proteins are related to melanin to decrease the color and quantity through cellular activation of lysosomes.

AB - As a high quantity of melanin can lead to hyperpigmentation or skin cancer in humans and it can also activate lysosomal enzymes so in the present study the changes in lysosomal enzymes upon exposure of HeLa cells to melanin has been studied. The HeLa cells were exposed to 100 ppm melanin for 24 hours and lysosomal enzymes were extracted. The lysosomal enzymes were identified through twodimensional polyacrylamide gel electrophoresis (2DE) after exposure of HeLa cells to melanin. The results showed 12 up and 3down regulation spots in relation to melanin exposure. It has been observed that lysosomal proteins are related to melanin to decrease the color and quantity through cellular activation of lysosomes.

KW - Lysosomal proteins

KW - Melanin

KW - Two-dimensional gel electrophoresis (2-DE)

UR - https://www.scopus.com/pages/publications/84957110108

U2 - 10.1007/s13530-015-0248-7

DO - 10.1007/s13530-015-0248-7

M3 - Journal article

AN - SCOPUS:84957110108

SN - 2005-9752

VL - 7

SP - 272

EP - 276

JO - Toxicology and Environmental Health Sciences

JF - Toxicology and Environmental Health Sciences

IS - 5

ER -

Different expression patterns of lysosomal proteins exposed to melanin in HeLa cells

Abstract

UN SDGs

Keywords

Quacquarelli Symonds(QS) Subject Topics

Access to Document

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