DNA/PEI nano-particles for gene delivery of rat bone marrow stem cells

Hyun Hee Ahn; Min Suk Lee; Mi Hee Cho; Yu Na Shin; Jung Hwa Lee; Kyung Sook Kim; Moon Suk Kim; Gilson Khang; Ki Chul Hwang; Il Woo Lee; Scott L. Diamond; Hai Bang Lee

doi:10.1016/j.colsurfa.2007.04.156

DNA/PEI nano-particles for gene delivery of rat bone marrow stem cells

Hyun Hee Ahn
, Min Suk Lee
, Mi Hee Cho
, Yu Na Shin
, Jung Hwa Lee
, Kyung Sook Kim
, Moon Suk Kim^*
, Gilson Khang
, Ki Chul Hwang
, Il Woo Lee
, Scott L. Diamond
, Hai Bang Lee

^*Corresponding author for this work

Department of Polymer -Nano Science &Technology

Korea Research Institute of Chemical Technology
The Catholic University of Korea
Jeonbuk National University
Yonsei University
University of Pennsylvania

Research output: Contribution to journal › Journal article › peer-review

31 Scopus citations

Abstract

The objective of this study was to deliver the DNA/PEI nano-particles into rat bone marrow stem cells (rBMSCs). The formation of nano-particles from DNA and PEI was performed by the adding of the PEI solution to the DNA solution. Gel retardation assay showed the optimal N/P charge ratio where PEI completely binds DNA. The particle size of DNA/PEI complexes measured by dynamic light scattering (DLS) showed diameters of about 200-400 nm. These results indicated that DNA was sufficiently condensed by PEI to give DNA/PEI nano-particles and the optimal packing was achieved at N/P charge ratio of 4. From AFM image, PEI completely condensed DNA into round-shaped. The diameters of the DNA/PEI nano-particles observed by AFM were in good agreement with the results of the DLS. rBMSCs viability in the presence of DNA/PEI nano-particles was evaluated by means of the MTT assay. The DNA/PEI nano-particles were transfected into rBMSCs. After gene transfection, enhanced green fluorescence protein (EGFP) was monitored by flow cytometry. Naked DNA showed no EGFP expression, whereas delivery of DNA/PEI nano-particles to rBMSCs showed EGFP expression and resulted in 2-10% transfection.

Original language	English
Pages (from-to)	116-120
Number of pages	5
Journal	Colloids and Surfaces A: Physicochemical and Engineering Aspects
Volume	313-314
DOIs	https://doi.org/10.1016/j.colsurfa.2007.04.156
State	Published - 2008.02.1

Keywords

Bone marrow stem cell
Gene delivery
Nano-particle
Transfection

Quacquarelli Symonds(QS) Subject Topics

Engineering - Petroleum
Engineering - Chemical
Chemistry
Physics & Astronomy

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10.1016/j.colsurfa.2007.04.156

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@article{00f323a0b15543198bd2f9536eb4aca5,

title = "DNA/PEI nano-particles for gene delivery of rat bone marrow stem cells",

abstract = "The objective of this study was to deliver the DNA/PEI nano-particles into rat bone marrow stem cells (rBMSCs). The formation of nano-particles from DNA and PEI was performed by the adding of the PEI solution to the DNA solution. Gel retardation assay showed the optimal N/P charge ratio where PEI completely binds DNA. The particle size of DNA/PEI complexes measured by dynamic light scattering (DLS) showed diameters of about 200-400 nm. These results indicated that DNA was sufficiently condensed by PEI to give DNA/PEI nano-particles and the optimal packing was achieved at N/P charge ratio of 4. From AFM image, PEI completely condensed DNA into round-shaped. The diameters of the DNA/PEI nano-particles observed by AFM were in good agreement with the results of the DLS. rBMSCs viability in the presence of DNA/PEI nano-particles was evaluated by means of the MTT assay. The DNA/PEI nano-particles were transfected into rBMSCs. After gene transfection, enhanced green fluorescence protein (EGFP) was monitored by flow cytometry. Naked DNA showed no EGFP expression, whereas delivery of DNA/PEI nano-particles to rBMSCs showed EGFP expression and resulted in 2-10\% transfection.",

keywords = "Bone marrow stem cell, Gene delivery, Nano-particle, Transfection",

author = "Ahn, \{Hyun Hee\} and Lee, \{Min Suk\} and Cho, \{Mi Hee\} and Shin, \{Yu Na\} and Lee, \{Jung Hwa\} and Kim, \{Kyung Sook\} and Kim, \{Moon Suk\} and Gilson Khang and Hwang, \{Ki Chul\} and Lee, \{Il Woo\} and Diamond, \{Scott L.\} and Lee, \{Hai Bang\}",

year = "2008",

month = feb,

day = "1",

doi = "10.1016/j.colsurfa.2007.04.156",

language = "English",

volume = "313-314",

pages = "116--120",

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TY - JOUR

T1 - DNA/PEI nano-particles for gene delivery of rat bone marrow stem cells

AU - Ahn, Hyun Hee

AU - Lee, Min Suk

AU - Cho, Mi Hee

AU - Shin, Yu Na

AU - Lee, Jung Hwa

AU - Kim, Kyung Sook

AU - Kim, Moon Suk

AU - Khang, Gilson

AU - Hwang, Ki Chul

AU - Lee, Il Woo

AU - Diamond, Scott L.

AU - Lee, Hai Bang

PY - 2008/2/1

Y1 - 2008/2/1

N2 - The objective of this study was to deliver the DNA/PEI nano-particles into rat bone marrow stem cells (rBMSCs). The formation of nano-particles from DNA and PEI was performed by the adding of the PEI solution to the DNA solution. Gel retardation assay showed the optimal N/P charge ratio where PEI completely binds DNA. The particle size of DNA/PEI complexes measured by dynamic light scattering (DLS) showed diameters of about 200-400 nm. These results indicated that DNA was sufficiently condensed by PEI to give DNA/PEI nano-particles and the optimal packing was achieved at N/P charge ratio of 4. From AFM image, PEI completely condensed DNA into round-shaped. The diameters of the DNA/PEI nano-particles observed by AFM were in good agreement with the results of the DLS. rBMSCs viability in the presence of DNA/PEI nano-particles was evaluated by means of the MTT assay. The DNA/PEI nano-particles were transfected into rBMSCs. After gene transfection, enhanced green fluorescence protein (EGFP) was monitored by flow cytometry. Naked DNA showed no EGFP expression, whereas delivery of DNA/PEI nano-particles to rBMSCs showed EGFP expression and resulted in 2-10% transfection.

AB - The objective of this study was to deliver the DNA/PEI nano-particles into rat bone marrow stem cells (rBMSCs). The formation of nano-particles from DNA and PEI was performed by the adding of the PEI solution to the DNA solution. Gel retardation assay showed the optimal N/P charge ratio where PEI completely binds DNA. The particle size of DNA/PEI complexes measured by dynamic light scattering (DLS) showed diameters of about 200-400 nm. These results indicated that DNA was sufficiently condensed by PEI to give DNA/PEI nano-particles and the optimal packing was achieved at N/P charge ratio of 4. From AFM image, PEI completely condensed DNA into round-shaped. The diameters of the DNA/PEI nano-particles observed by AFM were in good agreement with the results of the DLS. rBMSCs viability in the presence of DNA/PEI nano-particles was evaluated by means of the MTT assay. The DNA/PEI nano-particles were transfected into rBMSCs. After gene transfection, enhanced green fluorescence protein (EGFP) was monitored by flow cytometry. Naked DNA showed no EGFP expression, whereas delivery of DNA/PEI nano-particles to rBMSCs showed EGFP expression and resulted in 2-10% transfection.

KW - Bone marrow stem cell

KW - Gene delivery

KW - Nano-particle

KW - Transfection

UR - https://www.scopus.com/pages/publications/37349002391

U2 - 10.1016/j.colsurfa.2007.04.156

DO - 10.1016/j.colsurfa.2007.04.156

M3 - Journal article

AN - SCOPUS:37349002391

SN - 0927-7757

VL - 313-314

SP - 116

EP - 120

JO - Colloids and Surfaces A: Physicochemical and Engineering Aspects

JF - Colloids and Surfaces A: Physicochemical and Engineering Aspects

ER -

DNA/PEI nano-particles for gene delivery of rat bone marrow stem cells

Abstract

Keywords

Quacquarelli Symonds(QS) Subject Topics

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