Effect of cartilage regeneration on gellan gum and silk fibroin

Recently, in tissue engineering gellan gum as a polysaccharide composed of glucose, glutaric acid, and rham-nose is proposed as a substitute for cartilage regeneration applications. Silk fibroin has biocompatibility, biodegradability and excellent mechanical properties. In this work, to confirm the effect of silk fibroin on cartilage regeneration, hydrogel scaffolds were prepared by using 2 wt% silk fibroin (SF)/2 wt% gelln gum (GG) at weight ratio of 100/0, 70/30, and 50/50. The characterizations of scaffolds were implemented by FTIR, compressive strength, porosity, and SEM. Cell proliferation and gene expression were evaluated by MTT, bio-SEM, RT-PCR. As a result, SF50/GG50 scaffold was confirmed as an excellent scaffold for cartilage tissue regeneration.