Effect of silk sponge concentrations on skin regeneration

Silk is a well-studied biomaterial, possessing low immunity, high biocompatibility, and easy fabrication into various forms for several clinical applications. Herein, we have performed an in vitro (NIH/3T3 cells) and in vivo (rat models) study on silk sponges' efficacy for skin regulation. The silk sponges were fabricated with I, 2 and 3% silk contents via freeze-drying. All the samples physical characteristics were examined by FESEM and FT1R. In vitro biological properties including cell adhesion, proliferation and inflammation were confirmed by MTT assay and RT-PCR. Fur-Thermore, the skin regeneration efficiency was examined by histological assessment, where the silk sponges were implanted at full thickness skin wounds in rat models. Considering all the obtained results, the silk sponges can be envisioned as a potential biomaterial for wound healing applications.