Effect of SIS content on the proliferation of intervertebral disc cells of SIS/PLGA film

Porcine small intestine submucosa is accellular tissue and widely used as a biomaterials without immunorejection responses. Poly(lactide-co-glycilide) (PLGA) has controllable biodegradability and good biocompatibility. In this study, we evaluated 2-dimensional porcine small intestine submucosa (SIS)/PLGA hybrid films on adhesion, proliferation and phenotype maintenance of intervertebral disc cells. We prepared natural/synthetic hybrid films using 0, 10, 20, 40 and 80 wt% of SIS by the solvent evaporation method. SIS/PLGA films were characterized by scanning election microscopy (SEM). Disc cells were seeded on PLGA and SIS/PLGA film and we confirmed the effects of adhesion and proliferation on disc cells according to content of SIS by cell count and SEM observation. Contents of 10 wt% SIS positively affected to proliferate and maintain of disc phenotype in SIS/PLGA Film.