Effects of 7-MEGA^TM 500 on oxidative stress, inflammation, and skin regeneration in H₂O₂-treated skin cells

Environmental stimuli can lead to the excessive accumulation of reactive oxygen species (ROS), which is one of the risk factors for premature skin aging. Here, we investigated the protective effects of 7-MEGA^TM 500 (50% palmitoleic acid, 7-MEGA) against oxidative stress-induced cellular damage and its underlying therapeutic mechanisms in the HaCaT human skin keratinocyte cell line (HaCaT cells). Our results showed that treatment with 7-MEGA prior to hydrogen peroxide (H₂O₂)-induced damage significantly increased the viability of HaCaT cells. 7-MEGA effectively attenuated generation of H₂O₂-induced reactive oxygen species (ROS), and inhibited H₂O₂-induced inflammatory factors, such as prostaglandin E₂ (PGE₂), tumor necrosis factor-a (TNF-a), and interleukin-1ß (IL-1ß). In addition, cells treated with 7-MEGA exhibited significantly decreased expression of matrix metalloproteinase-1 (MMP-1) and increased expression of procollagen type 1 (PCOL1) and Elastin against oxidative stress by H₂O₂. Interestingly, these protective activities of 7-MEGA were similar in scope and of a higher magnitude than those seen with 98.5% palmitoleic acid (PA) obtained from Sigma when given at the same concentration (100 nL/mL). According to our data, 7-MEGA is able to protect HaCaT cells from H₂O₂-induced damage through inhibiting cellular oxidative stress and inflammation. Moreover, 7-MEGA may affect skin elasticity maintenance and improve skin wrinkles. These findings indicate that 7-MEGA may be useful as a food supplement for skin health.