Effects of Leucosporidium-derived ice-binding protein (LeIBP) on bull semen cryopreservation

Hoon Jang; Hyo J. Kwon; Wu S. Sun; Seongsoo Hwang; In S. Hwang; Sungwoo Kim; Jun H. Lee; Sung G. Lee; Jeong W. Lee

doi:10.1002/vms3.269

Effects of Leucosporidium-derived ice-binding protein (LeIBP) on bull semen cryopreservation

Hoon Jang
, Hyo J. Kwon
, Wu S. Sun
, Seongsoo Hwang
, In S. Hwang
, Sungwoo Kim
, Jun H. Lee
, Sung G. Lee
, Jeong W. Lee^*

^*Corresponding author for this work

Department of Biological Sciences

Korea Research Institute of Bioscience and Biotechnology
Rural Development Administration
Korea Polar Research Institute

Research output: Contribution to journal › Journal article › peer-review

10 Scopus citations

Abstract

We examined the effect of ice-binding protein derived from Leucosporidium (LeIBP) on the cryopreservation of bull semen and compared it with that derived from previously reported Antifreeze Protein III (AFPIII). Six concentrations of LeIBP (10^–1 ~ 10⁴ μg/ml) and AFPIII (10^–1 ~ 10⁴ μg/ml) were added to the bull semen extender, respectively. Sperm kinematic parameters were measured to examine sperm toxicity and cryopreserved sperm quality. Measures of antioxidant activity such as superoxide dismutase (SOD), reduced glutathione/oxidative glutathione (GSH/GSSG), and total antioxidant capacity (TAC) were analysed to identify the effect of LeIBP on sperm quality. In addition, sperm viability was analysed using a flow cytometer and fluorescence microscope by SYBR14/PI staining. The results showed that the LeIBP groups (0.1, 1 and 10 μg/ml) were less toxic, and the quality of the sperm were dramatically improved in the extenders containing 0.1 μg/ml LeIBP among concentrations of LeIBP and AFPIII. The SOD activity of LeIBP was greater than that of AFPIII and control. In addition, sperm viability was enhanced in the LeIBP-treated group. In summary, LeIBP is a useful cryoprotective adjuvant for bull sperm cryopreservation, and the most efficient concentration of LeIBP is 0.1 μg/ml.

Original language	English
Pages (from-to)	447-453
Number of pages	7
Journal	Veterinary Medicine and Science
Volume	6
Issue number	3
DOIs	https://doi.org/10.1002/vms3.269
State	Published - 2020.08.1

Keywords

Antioxidant activity
Bull sperm
Cryopreservation
LeIBP

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10.1002/vms3.269

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@article{e0058358f4f049b0a239e97f65713d5d,

title = "Effects of Leucosporidium-derived ice-binding protein (LeIBP) on bull semen cryopreservation",

abstract = "We examined the effect of ice-binding protein derived from Leucosporidium (LeIBP) on the cryopreservation of bull semen and compared it with that derived from previously reported Antifreeze Protein III (AFPIII). Six concentrations of LeIBP (10–1 \textasciitilde{} 104 μg/ml) and AFPIII (10–1 \textasciitilde{} 104 μg/ml) were added to the bull semen extender, respectively. Sperm kinematic parameters were measured to examine sperm toxicity and cryopreserved sperm quality. Measures of antioxidant activity such as superoxide dismutase (SOD), reduced glutathione/oxidative glutathione (GSH/GSSG), and total antioxidant capacity (TAC) were analysed to identify the effect of LeIBP on sperm quality. In addition, sperm viability was analysed using a flow cytometer and fluorescence microscope by SYBR14/PI staining. The results showed that the LeIBP groups (0.1, 1 and 10 μg/ml) were less toxic, and the quality of the sperm were dramatically improved in the extenders containing 0.1 μg/ml LeIBP among concentrations of LeIBP and AFPIII. The SOD activity of LeIBP was greater than that of AFPIII and control. In addition, sperm viability was enhanced in the LeIBP-treated group. In summary, LeIBP is a useful cryoprotective adjuvant for bull sperm cryopreservation, and the most efficient concentration of LeIBP is 0.1 μg/ml.",

keywords = "Antioxidant activity, Bull sperm, Cryopreservation, LeIBP",

author = "Hoon Jang and Kwon, \{Hyo J.\} and Sun, \{Wu S.\} and Seongsoo Hwang and Hwang, \{In S.\} and Sungwoo Kim and Lee, \{Jun H.\} and Lee, \{Sung G.\} and Lee, \{Jeong W.\}",

note = "Publisher Copyright: {\textcopyright} 2020 The Authors. Veterinary Medicine and Science Published by John Wiley \& Sons Ltd",

year = "2020",

month = aug,

day = "1",

doi = "10.1002/vms3.269",

language = "English",

volume = "6",

pages = "447--453",

journal = "Veterinary Medicine and Science",

issn = "2053-1095",

number = "3",

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TY - JOUR

T1 - Effects of Leucosporidium-derived ice-binding protein (LeIBP) on bull semen cryopreservation

AU - Jang, Hoon

AU - Kwon, Hyo J.

AU - Sun, Wu S.

AU - Hwang, Seongsoo

AU - Hwang, In S.

AU - Kim, Sungwoo

AU - Lee, Jun H.

AU - Lee, Sung G.

AU - Lee, Jeong W.

PY - 2020/8/1

Y1 - 2020/8/1

N2 - We examined the effect of ice-binding protein derived from Leucosporidium (LeIBP) on the cryopreservation of bull semen and compared it with that derived from previously reported Antifreeze Protein III (AFPIII). Six concentrations of LeIBP (10–1 ~ 104 μg/ml) and AFPIII (10–1 ~ 104 μg/ml) were added to the bull semen extender, respectively. Sperm kinematic parameters were measured to examine sperm toxicity and cryopreserved sperm quality. Measures of antioxidant activity such as superoxide dismutase (SOD), reduced glutathione/oxidative glutathione (GSH/GSSG), and total antioxidant capacity (TAC) were analysed to identify the effect of LeIBP on sperm quality. In addition, sperm viability was analysed using a flow cytometer and fluorescence microscope by SYBR14/PI staining. The results showed that the LeIBP groups (0.1, 1 and 10 μg/ml) were less toxic, and the quality of the sperm were dramatically improved in the extenders containing 0.1 μg/ml LeIBP among concentrations of LeIBP and AFPIII. The SOD activity of LeIBP was greater than that of AFPIII and control. In addition, sperm viability was enhanced in the LeIBP-treated group. In summary, LeIBP is a useful cryoprotective adjuvant for bull sperm cryopreservation, and the most efficient concentration of LeIBP is 0.1 μg/ml.

AB - We examined the effect of ice-binding protein derived from Leucosporidium (LeIBP) on the cryopreservation of bull semen and compared it with that derived from previously reported Antifreeze Protein III (AFPIII). Six concentrations of LeIBP (10–1 ~ 104 μg/ml) and AFPIII (10–1 ~ 104 μg/ml) were added to the bull semen extender, respectively. Sperm kinematic parameters were measured to examine sperm toxicity and cryopreserved sperm quality. Measures of antioxidant activity such as superoxide dismutase (SOD), reduced glutathione/oxidative glutathione (GSH/GSSG), and total antioxidant capacity (TAC) were analysed to identify the effect of LeIBP on sperm quality. In addition, sperm viability was analysed using a flow cytometer and fluorescence microscope by SYBR14/PI staining. The results showed that the LeIBP groups (0.1, 1 and 10 μg/ml) were less toxic, and the quality of the sperm were dramatically improved in the extenders containing 0.1 μg/ml LeIBP among concentrations of LeIBP and AFPIII. The SOD activity of LeIBP was greater than that of AFPIII and control. In addition, sperm viability was enhanced in the LeIBP-treated group. In summary, LeIBP is a useful cryoprotective adjuvant for bull sperm cryopreservation, and the most efficient concentration of LeIBP is 0.1 μg/ml.

KW - Antioxidant activity

KW - Bull sperm

KW - Cryopreservation

KW - LeIBP

UR - https://www.scopus.com/pages/publications/85083764894

U2 - 10.1002/vms3.269

DO - 10.1002/vms3.269

M3 - Journal article

C2 - 32323490

AN - SCOPUS:85083764894

SN - 2053-1095

VL - 6

SP - 447

EP - 453

JO - Veterinary Medicine and Science

JF - Veterinary Medicine and Science

IS - 3

ER -

Effects of Leucosporidium-derived ice-binding protein (LeIBP) on bull semen cryopreservation

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Keywords

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