Epigallocatechin-3-gallate, constituent of green tea, suppresses the LPS-induced phenotypic and functional maturation of murine dendritic cells through inhibition of mitogen-activated protein kinases and NF-κB

Soon Cheol Ahn; Gi Young Kim; Jin Hyung Kim; Seong Wan Baik; Myung Kwan Han; Hee Jeong Lee; Dong Oh Moon; Chang Min Lee; Ju Hyung Kang; Bo Hye Kim; Yang Hyo Oh; Yeong Min Park

doi:10.1016/j.bbrc.2003.11.108

Epigallocatechin-3-gallate, constituent of green tea, suppresses the LPS-induced phenotypic and functional maturation of murine dendritic cells through inhibition of mitogen-activated protein kinases and NF-κB

Soon Cheol Ahn
, Gi Young Kim
, Jin Hyung Kim
, Seong Wan Baik
, Myung Kwan Han
, Hee Jeong Lee
, Dong Oh Moon
, Chang Min Lee
, Ju Hyung Kang
, Bo Hye Kim
, Yang Hyo Oh
, Yeong Min Park^*

^*Corresponding author for this work

Department of Medicine

Pusan National University
Dong-A University

Research output: Contribution to journal › Journal article › peer-review

104 Scopus citations

Abstract

The effects of epigallocatechin-3-gallate (EGCG) on dendritic cells (DC) maturation were investigated. EGCG, in a dose-dependent manner, profoundly inhibited CD80, CD86, and MHC class I and II expression on bone marrow-derived murine myeloid DC. EGCG restored the decreased dextran-FITC uptake and inhibited enhanced IL-12 production by LPS-treated DC. EGCG-treated DC were poor stimulators of naïve allogeneic T-cell proliferation and reduced levels of IL-2 production in responding T cells. EGCG-pretreated DC inhibited LPS-induced MAPKs, such as ERK1/2, p38, JNK, and NF-κB p65 translocation. Therefore, the molecular mechanisms by which EGCG antagonized LPS-induced DC maturation appeared to involve the inhibition of MAPK and NF-κB activation. These novel findings provide new insight into the immunopharmacological role of EGCG and suggest a novel approach to the manipulation of DC for therapeutic application of autoimmune and allergic diseases.

Original language	English
Pages (from-to)	148-155
Number of pages	8
Journal	Biochemical and Biophysical Research Communications
Volume	313
Issue number	1
DOIs	https://doi.org/10.1016/j.bbrc.2003.11.108
State	Published - 2004.01.2

Keywords

Dendritic cell
Epigallocatechin-3-gallate
Mitogen-activated protein kinases
NF-κB

Quacquarelli Symonds(QS) Subject Topics

Biological Sciences

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Ahn, S. C., Kim, G. Y., Kim, J. H., Baik, S. W., Han, M. K., Lee, H. J., Moon, D. O., Lee, C. M., Kang, J. H., Kim, B. H., Oh, Y. H., & Park, Y. M. (2004). Epigallocatechin-3-gallate, constituent of green tea, suppresses the LPS-induced phenotypic and functional maturation of murine dendritic cells through inhibition of mitogen-activated protein kinases and NF-κB. Biochemical and Biophysical Research Communications, 313(1), 148-155. https://doi.org/10.1016/j.bbrc.2003.11.108

@article{bead01a11b4c4a368a8b2f2d2ecc75b4,

title = "Epigallocatechin-3-gallate, constituent of green tea, suppresses the LPS-induced phenotypic and functional maturation of murine dendritic cells through inhibition of mitogen-activated protein kinases and NF-κB",

abstract = "The effects of epigallocatechin-3-gallate (EGCG) on dendritic cells (DC) maturation were investigated. EGCG, in a dose-dependent manner, profoundly inhibited CD80, CD86, and MHC class I and II expression on bone marrow-derived murine myeloid DC. EGCG restored the decreased dextran-FITC uptake and inhibited enhanced IL-12 production by LPS-treated DC. EGCG-treated DC were poor stimulators of na{\"i}ve allogeneic T-cell proliferation and reduced levels of IL-2 production in responding T cells. EGCG-pretreated DC inhibited LPS-induced MAPKs, such as ERK1/2, p38, JNK, and NF-κB p65 translocation. Therefore, the molecular mechanisms by which EGCG antagonized LPS-induced DC maturation appeared to involve the inhibition of MAPK and NF-κB activation. These novel findings provide new insight into the immunopharmacological role of EGCG and suggest a novel approach to the manipulation of DC for therapeutic application of autoimmune and allergic diseases.",

keywords = "Dendritic cell, Epigallocatechin-3-gallate, Mitogen-activated protein kinases, NF-κB",

author = "Ahn, \{Soon Cheol\} and Kim, \{Gi Young\} and Kim, \{Jin Hyung\} and Baik, \{Seong Wan\} and Han, \{Myung Kwan\} and Lee, \{Hee Jeong\} and Moon, \{Dong Oh\} and Lee, \{Chang Min\} and Kang, \{Ju Hyung\} and Kim, \{Bo Hye\} and Oh, \{Yang Hyo\} and Park, \{Yeong Min\}",

year = "2004",

month = jan,

day = "2",

doi = "10.1016/j.bbrc.2003.11.108",

language = "English",

volume = "313",

pages = "148--155",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

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Ahn, SC, Kim, GY, Kim, JH, Baik, SW, Han, MK, Lee, HJ, Moon, DO, Lee, CM, Kang, JH, Kim, BH, Oh, YH & Park, YM 2004, 'Epigallocatechin-3-gallate, constituent of green tea, suppresses the LPS-induced phenotypic and functional maturation of murine dendritic cells through inhibition of mitogen-activated protein kinases and NF-κB', Biochemical and Biophysical Research Communications, vol. 313, no. 1, pp. 148-155. https://doi.org/10.1016/j.bbrc.2003.11.108

Epigallocatechin-3-gallate, constituent of green tea, suppresses the LPS-induced phenotypic and functional maturation of murine dendritic cells through inhibition of mitogen-activated protein kinases and NF-κB. / Ahn, Soon Cheol; Kim, Gi Young; Kim, Jin Hyung et al.
In: Biochemical and Biophysical Research Communications, Vol. 313, No. 1, 02.01.2004, p. 148-155.

Research output: Contribution to journal › Journal article › peer-review

TY - JOUR

T1 - Epigallocatechin-3-gallate, constituent of green tea, suppresses the LPS-induced phenotypic and functional maturation of murine dendritic cells through inhibition of mitogen-activated protein kinases and NF-κB

AU - Ahn, Soon Cheol

AU - Kim, Gi Young

AU - Kim, Jin Hyung

AU - Baik, Seong Wan

AU - Han, Myung Kwan

AU - Lee, Hee Jeong

AU - Moon, Dong Oh

AU - Lee, Chang Min

AU - Kang, Ju Hyung

AU - Kim, Bo Hye

AU - Oh, Yang Hyo

AU - Park, Yeong Min

PY - 2004/1/2

Y1 - 2004/1/2

N2 - The effects of epigallocatechin-3-gallate (EGCG) on dendritic cells (DC) maturation were investigated. EGCG, in a dose-dependent manner, profoundly inhibited CD80, CD86, and MHC class I and II expression on bone marrow-derived murine myeloid DC. EGCG restored the decreased dextran-FITC uptake and inhibited enhanced IL-12 production by LPS-treated DC. EGCG-treated DC were poor stimulators of naïve allogeneic T-cell proliferation and reduced levels of IL-2 production in responding T cells. EGCG-pretreated DC inhibited LPS-induced MAPKs, such as ERK1/2, p38, JNK, and NF-κB p65 translocation. Therefore, the molecular mechanisms by which EGCG antagonized LPS-induced DC maturation appeared to involve the inhibition of MAPK and NF-κB activation. These novel findings provide new insight into the immunopharmacological role of EGCG and suggest a novel approach to the manipulation of DC for therapeutic application of autoimmune and allergic diseases.

AB - The effects of epigallocatechin-3-gallate (EGCG) on dendritic cells (DC) maturation were investigated. EGCG, in a dose-dependent manner, profoundly inhibited CD80, CD86, and MHC class I and II expression on bone marrow-derived murine myeloid DC. EGCG restored the decreased dextran-FITC uptake and inhibited enhanced IL-12 production by LPS-treated DC. EGCG-treated DC were poor stimulators of naïve allogeneic T-cell proliferation and reduced levels of IL-2 production in responding T cells. EGCG-pretreated DC inhibited LPS-induced MAPKs, such as ERK1/2, p38, JNK, and NF-κB p65 translocation. Therefore, the molecular mechanisms by which EGCG antagonized LPS-induced DC maturation appeared to involve the inhibition of MAPK and NF-κB activation. These novel findings provide new insight into the immunopharmacological role of EGCG and suggest a novel approach to the manipulation of DC for therapeutic application of autoimmune and allergic diseases.

KW - Dendritic cell

KW - Epigallocatechin-3-gallate

KW - Mitogen-activated protein kinases

KW - NF-κB

UR - https://www.scopus.com/pages/publications/0348111402

U2 - 10.1016/j.bbrc.2003.11.108

DO - 10.1016/j.bbrc.2003.11.108

M3 - Journal article

C2 - 14672711

AN - SCOPUS:0348111402

SN - 0006-291X

VL - 313

SP - 148

EP - 155

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 1

ER -

Ahn SC, Kim GY, Kim JH, Baik SW, Han MK, Lee HJ et al. Epigallocatechin-3-gallate, constituent of green tea, suppresses the LPS-induced phenotypic and functional maturation of murine dendritic cells through inhibition of mitogen-activated protein kinases and NF-κB. Biochemical and Biophysical Research Communications. 2004 Jan 2;313(1):148-155. doi: 10.1016/j.bbrc.2003.11.108

Epigallocatechin-3-gallate, constituent of green tea, suppresses the LPS-induced phenotypic and functional maturation of murine dendritic cells through inhibition of mitogen-activated protein kinases and NF-κB

Abstract

Keywords

Quacquarelli Symonds(QS) Subject Topics

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