Erratum to Production of isobutanol from crude glycerol by a genetically-engineered Klebsiella pneumoniae strain

Baek Rock Oh; Sun Yeon Heo; Sung Mok Lee; Won Kyung Hong; Jang Min Park; You Ree Jung; Dae Hyuk Kim; Jung Hoon Sohn; Jeong Woo Seo; Chul Ho Kim

doi:10.1007/s10529-013-1383-3

Erratum to Production of isobutanol from crude glycerol by a genetically-engineered Klebsiella pneumoniae strain

Baek Rock Oh
, Sun Yeon Heo
, Sung Mok Lee
, Won Kyung Hong
, Jang Min Park
, You Ree Jung
, Dae Hyuk Kim
, Jung Hoon Sohn
, Jeong Woo Seo
, Chul Ho Kim

Department of Molecular Biology

Korea Research Institute of Bioscience and Biotechnology

Research output: Contribution to journal › Comment/debate

8 Scopus citations

Abstract

Klebsiella pneumoniae was engineered to produce isobutanol from crude glycerol as a sole carbon source by expressing acetolactate synthase (ilvIH), keto-acid reducto-isomerase (ilvC) and dihydroxy-acid dehydratase (ilvD) from K. pneumoniae, and α-ketoisovalerate decarboxylase (kivd) and alcohol dehydrogenase (adhA) from Lactococcus lactis. Engineered K. pneumonia, {increment}ldhA/pBR-iBO (ilvIH-ilvC-ilvD-kivd-adhA), produced isobutanol (160 mg l^-1) from crude glycerol. To increase the yield of isobutanol, we eliminated the 2,3-butanediol pathway from the recombinant strain by inactivating α-acetolactate decarboxylase (adc). This further engineering step improved the yield of isobutanol from 160 to 320 mg l^-1. This represents the first successful attempt to produce isobutanol from crude glycerol.

Original language	English
Pages (from-to)	397-402
Number of pages	6
Journal	Biotechnology Letters
Volume	36
Issue number	2
DOIs	https://doi.org/10.1007/s10529-013-1383-3
State	Published - 2014.02

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Crude glycerol
Genetic engineering
Isobutanol
Klebsiella pneumoniae

Quacquarelli Symonds(QS) Subject Topics

Engineering - Chemical
Biological Sciences

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10.1007/s10529-013-1383-3

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title = "Erratum to Production of isobutanol from crude glycerol by a genetically-engineered Klebsiella pneumoniae strain",

abstract = "Klebsiella pneumoniae was engineered to produce isobutanol from crude glycerol as a sole carbon source by expressing acetolactate synthase (ilvIH), keto-acid reducto-isomerase (ilvC) and dihydroxy-acid dehydratase (ilvD) from K. pneumoniae, and α-ketoisovalerate decarboxylase (kivd) and alcohol dehydrogenase (adhA) from Lactococcus lactis. Engineered K. pneumonia, \{increment\}ldhA/pBR-iBO (ilvIH-ilvC-ilvD-kivd-adhA), produced isobutanol (160 mg l-1) from crude glycerol. To increase the yield of isobutanol, we eliminated the 2,3-butanediol pathway from the recombinant strain by inactivating α-acetolactate decarboxylase (adc). This further engineering step improved the yield of isobutanol from 160 to 320 mg l-1. This represents the first successful attempt to produce isobutanol from crude glycerol.",

keywords = "Crude glycerol, Genetic engineering, Isobutanol, Klebsiella pneumoniae",

author = "Oh, \{Baek Rock\} and Heo, \{Sun Yeon\} and Lee, \{Sung Mok\} and Hong, \{Won Kyung\} and Park, \{Jang Min\} and Jung, \{You Ree\} and Kim, \{Dae Hyuk\} and Sohn, \{Jung Hoon\} and Seo, \{Jeong Woo\} and Kim, \{Chul Ho\}",

year = "2014",

month = feb,

doi = "10.1007/s10529-013-1383-3",

language = "English",

volume = "36",

pages = "397--402",

journal = "Biotechnology Letters",

issn = "0141-5492",

number = "2",

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TY - JOUR

T1 - Erratum to Production of isobutanol from crude glycerol by a genetically-engineered Klebsiella pneumoniae strain

AU - Oh, Baek Rock

AU - Heo, Sun Yeon

AU - Lee, Sung Mok

AU - Hong, Won Kyung

AU - Park, Jang Min

AU - Jung, You Ree

AU - Kim, Dae Hyuk

AU - Sohn, Jung Hoon

AU - Seo, Jeong Woo

AU - Kim, Chul Ho

PY - 2014/2

Y1 - 2014/2

N2 - Klebsiella pneumoniae was engineered to produce isobutanol from crude glycerol as a sole carbon source by expressing acetolactate synthase (ilvIH), keto-acid reducto-isomerase (ilvC) and dihydroxy-acid dehydratase (ilvD) from K. pneumoniae, and α-ketoisovalerate decarboxylase (kivd) and alcohol dehydrogenase (adhA) from Lactococcus lactis. Engineered K. pneumonia, {increment}ldhA/pBR-iBO (ilvIH-ilvC-ilvD-kivd-adhA), produced isobutanol (160 mg l-1) from crude glycerol. To increase the yield of isobutanol, we eliminated the 2,3-butanediol pathway from the recombinant strain by inactivating α-acetolactate decarboxylase (adc). This further engineering step improved the yield of isobutanol from 160 to 320 mg l-1. This represents the first successful attempt to produce isobutanol from crude glycerol.

AB - Klebsiella pneumoniae was engineered to produce isobutanol from crude glycerol as a sole carbon source by expressing acetolactate synthase (ilvIH), keto-acid reducto-isomerase (ilvC) and dihydroxy-acid dehydratase (ilvD) from K. pneumoniae, and α-ketoisovalerate decarboxylase (kivd) and alcohol dehydrogenase (adhA) from Lactococcus lactis. Engineered K. pneumonia, {increment}ldhA/pBR-iBO (ilvIH-ilvC-ilvD-kivd-adhA), produced isobutanol (160 mg l-1) from crude glycerol. To increase the yield of isobutanol, we eliminated the 2,3-butanediol pathway from the recombinant strain by inactivating α-acetolactate decarboxylase (adc). This further engineering step improved the yield of isobutanol from 160 to 320 mg l-1. This represents the first successful attempt to produce isobutanol from crude glycerol.

KW - Crude glycerol

KW - Genetic engineering

KW - Isobutanol

KW - Klebsiella pneumoniae

UR - https://www.scopus.com/pages/publications/84892525283

U2 - 10.1007/s10529-013-1383-3

DO - 10.1007/s10529-013-1383-3

M3 - Comment/debate

AN - SCOPUS:84892525283

SN - 0141-5492

VL - 36

SP - 397

EP - 402

JO - Biotechnology Letters

JF - Biotechnology Letters

IS - 2

ER -

Erratum to Production of isobutanol from crude glycerol by a genetically-engineered Klebsiella pneumoniae strain

Abstract

UN SDGs

Keywords

Quacquarelli Symonds(QS) Subject Topics

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