Evaluation of double network hydrogel of poloxamer-heparin/gellan gum for bone marrow stem cells delivery carrier

Joo Hee Choi; Ok Kyun Choi; Jeonghun Lee; Joungyoun Noh; Sumi Lee; Ain Park; Min A. Rim; Rui L. Reis; Gilson Khang

doi:10.1016/j.colsurfb.2019.06.041

Evaluation of double network hydrogel of poloxamer-heparin/gellan gum for bone marrow stem cells delivery carrier

Joo Hee Choi
, Ok Kyun Choi
, Jeonghun Lee
, Joungyoun Noh
, Sumi Lee
, Ain Park
, Min A. Rim
, Rui L. Reis
, Gilson Khang^*

^*Corresponding author for this work

Department of Polymer -Nano Science &Technology

Jeonbuk National University
University of Minho

Research output: Contribution to journal › Journal article › peer-review

40 Scopus citations

Abstract

In this study, a double network hydrogel of a natural polysaccharide gellan gum (GG) hydrogel and a synthetic hydrogel poloxamer-heparin (PoH) hydrogel (PoH/GG DNH) is introduced to complement disadvantages of each hydrogel and improve the microenvironment for cell delivery. The microstructure, surface morphology, gelation temperature, swelling and weight loss, sol fraction, mechanical property and thermal stability was examined. The potential of the composite hydrogel for cell vehicle was demonstrated by encapsulation of bone marrow stem cells isolated from rabbits (rBMSCs) within the PoH/GG DNH in vitro. The results showed that the DNH system supported cell survival and retained rBMSCs morphology and phenotype. Moreover, cell distribution, adherence, and ECM production were supported by PoH/GG DNH in vivo. Overall results provide a potential opportunity to apply the composite hydrogels in tissue engineering purpose.

Original language	English
Pages (from-to)	879-889
Number of pages	11
Journal	Colloids and Surfaces B: Biointerfaces
Volume	181
DOIs	https://doi.org/10.1016/j.colsurfb.2019.06.041
State	Published - 2019.09.1

Keywords

Bone marrow stem cells
Double network hydrogel
Hydrogel
Nature-derived
Regeneration
Synthetic

Quacquarelli Symonds(QS) Subject Topics

Engineering - Petroleum
Engineering - Chemical
Chemistry
Physics & Astronomy
Biological Sciences

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10.1016/j.colsurfb.2019.06.041

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@article{eebf8bbdf7da4214a3448aa7fe3b9b2c,

title = "Evaluation of double network hydrogel of poloxamer-heparin/gellan gum for bone marrow stem cells delivery carrier",

abstract = "In this study, a double network hydrogel of a natural polysaccharide gellan gum (GG) hydrogel and a synthetic hydrogel poloxamer-heparin (PoH) hydrogel (PoH/GG DNH) is introduced to complement disadvantages of each hydrogel and improve the microenvironment for cell delivery. The microstructure, surface morphology, gelation temperature, swelling and weight loss, sol fraction, mechanical property and thermal stability was examined. The potential of the composite hydrogel for cell vehicle was demonstrated by encapsulation of bone marrow stem cells isolated from rabbits (rBMSCs) within the PoH/GG DNH in vitro. The results showed that the DNH system supported cell survival and retained rBMSCs morphology and phenotype. Moreover, cell distribution, adherence, and ECM production were supported by PoH/GG DNH in vivo. Overall results provide a potential opportunity to apply the composite hydrogels in tissue engineering purpose.",

keywords = "Bone marrow stem cells, Double network hydrogel, Hydrogel, Nature-derived, Regeneration, Synthetic",

author = "Choi, \{Joo Hee\} and Choi, \{Ok Kyun\} and Jeonghun Lee and Joungyoun Noh and Sumi Lee and Ain Park and Rim, \{Min A.\} and Reis, \{Rui L.\} and Gilson Khang",

note = "Publisher Copyright: {\textcopyright} 2019",

year = "2019",

month = sep,

day = "1",

doi = "10.1016/j.colsurfb.2019.06.041",

language = "English",

volume = "181",

pages = "879--889",

journal = "Colloids and Surfaces B: Biointerfaces",

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TY - JOUR

T1 - Evaluation of double network hydrogel of poloxamer-heparin/gellan gum for bone marrow stem cells delivery carrier

AU - Choi, Joo Hee

AU - Choi, Ok Kyun

AU - Lee, Jeonghun

AU - Noh, Joungyoun

AU - Lee, Sumi

AU - Park, Ain

AU - Rim, Min A.

AU - Reis, Rui L.

AU - Khang, Gilson

PY - 2019/9/1

Y1 - 2019/9/1

N2 - In this study, a double network hydrogel of a natural polysaccharide gellan gum (GG) hydrogel and a synthetic hydrogel poloxamer-heparin (PoH) hydrogel (PoH/GG DNH) is introduced to complement disadvantages of each hydrogel and improve the microenvironment for cell delivery. The microstructure, surface morphology, gelation temperature, swelling and weight loss, sol fraction, mechanical property and thermal stability was examined. The potential of the composite hydrogel for cell vehicle was demonstrated by encapsulation of bone marrow stem cells isolated from rabbits (rBMSCs) within the PoH/GG DNH in vitro. The results showed that the DNH system supported cell survival and retained rBMSCs morphology and phenotype. Moreover, cell distribution, adherence, and ECM production were supported by PoH/GG DNH in vivo. Overall results provide a potential opportunity to apply the composite hydrogels in tissue engineering purpose.

AB - In this study, a double network hydrogel of a natural polysaccharide gellan gum (GG) hydrogel and a synthetic hydrogel poloxamer-heparin (PoH) hydrogel (PoH/GG DNH) is introduced to complement disadvantages of each hydrogel and improve the microenvironment for cell delivery. The microstructure, surface morphology, gelation temperature, swelling and weight loss, sol fraction, mechanical property and thermal stability was examined. The potential of the composite hydrogel for cell vehicle was demonstrated by encapsulation of bone marrow stem cells isolated from rabbits (rBMSCs) within the PoH/GG DNH in vitro. The results showed that the DNH system supported cell survival and retained rBMSCs morphology and phenotype. Moreover, cell distribution, adherence, and ECM production were supported by PoH/GG DNH in vivo. Overall results provide a potential opportunity to apply the composite hydrogels in tissue engineering purpose.

KW - Bone marrow stem cells

KW - Double network hydrogel

KW - Hydrogel

KW - Nature-derived

KW - Regeneration

KW - Synthetic

UR - https://www.scopus.com/pages/publications/85067973952

U2 - 10.1016/j.colsurfb.2019.06.041

DO - 10.1016/j.colsurfb.2019.06.041

M3 - Journal article

C2 - 31382336

AN - SCOPUS:85067973952

SN - 0927-7765

VL - 181

SP - 879

EP - 889

JO - Colloids and Surfaces B: Biointerfaces

JF - Colloids and Surfaces B: Biointerfaces

ER -

Evaluation of double network hydrogel of poloxamer-heparin/gellan gum for bone marrow stem cells delivery carrier

Abstract

Keywords

Quacquarelli Symonds(QS) Subject Topics

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